Table 2.

CDR3 junctional sequences and design of ASOs for each patient

Case
5′-VH
N-DH-N
JH-3′
DH
JH-3′
Taa
1  TGTGCGAGAGA tcctGATATTGTAGTAGTACCAGCTGCTATgaattactactactacggt   ATGGACGTC   D2-2   JH6   60  
2   GCGAGAGA   gcaggctatcGGGTATAGCAGgcc CTTTGACTAC   D6-25   JH4   60  
3   GCGAGAG   tcgccggaacgcgagaagTACGATTTTTGGAGTGGTTATactcaaagggcgcgt   ACTACTTTGACTAC   D3-3   JH4   60  
4   GCGAGAGG   ctggctaggatattgtagtagtaccagctgcgcagtggggacGTATTACGATTTTTGGAGTGGTTATTcctcaact   TTTGACTAC   D3-3   JH4   60  
5  TGTGCACGGATAC caGATTTTTGGAGTGCTTATTccaccgtgtaa   GTACTTCGATCTC   D3-3   JH2   60  
6   TGTGCGAGA cgAGTGCGAAGT  ACAACTGGTTCGACCCC   D1-26   JH5   60  
7   GCG   ctcccctcgGGATATAGTGCCTACAATT   ACTACTTTGACTAC   D5-12   JH4   60  
8   GCGTTAG  gtccattaggGTATTACGATTTTTGGAGTGGTTATCAACCCaaatactttgg   CTACGGTATGGACGTC   D3-3   JH6   60  
9   GCG   agaggatgggGCAGCACTTGGGAagtacgaaga  TACTACTACTACGGTATGGACGTC   D6-13   JH5   60  
10  TGTGCGAGAGA tcagTGGCTGCCTATTAat   TACTTTGACTAC   D3-9   JH4   60  
11  GCGAGAG ggACGGTTCGGGGAGTTATTgggttatacttggactactacggtatg   GACGTC   D3-10   JH6   60  
12   TGTGCGAGAG cTAGTGGGAG CTATGACTAC   D1-26   JH4   60  
13   GCAAGAGA ccgGTATTACGATTTTTGGAGTGGTTATTATgtac   TTGACTAC   D3-3   JH4   65  
14   GCGAGA  cagcagttcgccccaTATTACGATTTTTGGAGTGGTTcctcttttcgatt   CTACTACTACGGTA   D3-3   JH6   60  
15   GCGAGAG   tcgcgggagtcGCATATTGTGGTGGTGACTGCTATTggagagagt   ACTACTTTGACTAC   D2-21   JH4   60  
16   GCGAGA  gatccccGCCATAGCAGCAGCTGGTACccctactactactacggtatg   GACGCT   D6-13   JH6   60  
17   GCGAACG   gTGTCAGGGGTGACTACgtgtatccg TCCGGTATGGACGTC   D4-17   JH6   60  
18  GCGAGACA tcgactAGGATATTGTAGTAGTACCAGCTGC   TACTACTACTACTACGGTA   D2-2   JH6   60  
19   GCGAAAGA   ttcggggtatcaGTATTACGATTTTTGGAGTGGTTATTATAacgata   ATTACTACTACTACTACGGTA   D3-3   JH6   60  
20   GCGAG gaaggagtcTTACGATTTTTGGAGTGGTTATTtgtcccgt   TACTACTACTACGGTA   D3-3   JH6   60  
21   GCGAGA actttgagaattccGTATTACTATGATAGTAGTGGTTATTATTggggct   ACAAC   D3-22   JH5   60  
22   GCG   agacggggGGGTATTGTAGTGGTGGTAGCTGCCATT TGGGACTAC   D2-15   JH4   60  
23   GCG   agatggtctttcggtTATTGTGCTGGTGGCATCTGttacggtggaaactaccaatactacgctatg   GACGTC   D2-15   JH6   60  
24   GCGAG   ccgcGATATTGTAGTGGTGGTAGCTGCTACCCCgg   AATACTACTACTACATGGACGTC   D2-15   JH6   66  
25  TGTATTACTGTGC cgtaTGTGGTGGTTATTACTATGC   TGACTAC   D2-21   JH4   60  
26  CTGTGCGAAAGA tagggatgACTATGGTaattacgt   CTTTGACTAC   D3-10   JH4   60  
27   GTGAGAGA  tacctccaGTATAGTAGGAGGTGGTCCccttgaccgt   TGG   D6-13   JH5   60  
28   GCAAGA   gatgcttggcggcccgctcgccccgcGTATTATGATTACGTTTGGGGGAGTTATCGCCAcggccccca   TGATGCTTTTGATATC   D3-16   JH3   60  
29   ACCACAG   cccgtcggtgcttgcgccccCGATATTTTGACTGGTTATTGGACCcgggggta   TGCTACTACGGTATGGACGTC   D3-9   JH6   60  
30   GCGAGA   gattcccacttGTATTACGATATTTTGACTGGTTATTCTActgaaatggag  GACTAC   D3-9   JH4   60  
31   GCGAGAGA tcgccccggGGGTATTGTAGTAGTACCAGCTGCTATGtgctactacggta   TGGACGTC   D2-2   JH6   60  
32   GCGGCCG cTGCAGATTTTTGGAGTGGTTCTa   AAGAC   D3-3   JH4   60  
33  TGCGAGTG gTTACTATGATTCGGGGAc   CTACTACGGTGTGGACGTC   D3-10   JH6   60  
34   GCAAAAGAT   tctgttcagatgAGCAACCCCTGGTACgaaggaggggg   CTTCTGGTTCGACCCC   D6-13   JH5   60  
35   GCGAGAAA   accaagtTATTACTATGATAGTAGTGGTTATTACTACtgga   ACTACTACTACTACGGTATGGACGTC  D3-22   JH6   NF  
36
 		 GCGAAAG
 		 gtgctagTTACTTTGGTTCGGGGAGTTTTTATAACgagcc
 		CTCTGACCACTGG
 		 D3-10
 		 JH4
 		 NF
 
Case
5′-VH
N-DH-N
JH-3′
DH
JH-3′
Taa
1  TGTGCGAGAGA tcctGATATTGTAGTAGTACCAGCTGCTATgaattactactactacggt   ATGGACGTC   D2-2   JH6   60  
2   GCGAGAGA   gcaggctatcGGGTATAGCAGgcc CTTTGACTAC   D6-25   JH4   60  
3   GCGAGAG   tcgccggaacgcgagaagTACGATTTTTGGAGTGGTTATactcaaagggcgcgt   ACTACTTTGACTAC   D3-3   JH4   60  
4   GCGAGAGG   ctggctaggatattgtagtagtaccagctgcgcagtggggacGTATTACGATTTTTGGAGTGGTTATTcctcaact   TTTGACTAC   D3-3   JH4   60  
5  TGTGCACGGATAC caGATTTTTGGAGTGCTTATTccaccgtgtaa   GTACTTCGATCTC   D3-3   JH2   60  
6   TGTGCGAGA cgAGTGCGAAGT  ACAACTGGTTCGACCCC   D1-26   JH5   60  
7   GCG   ctcccctcgGGATATAGTGCCTACAATT   ACTACTTTGACTAC   D5-12   JH4   60  
8   GCGTTAG  gtccattaggGTATTACGATTTTTGGAGTGGTTATCAACCCaaatactttgg   CTACGGTATGGACGTC   D3-3   JH6   60  
9   GCG   agaggatgggGCAGCACTTGGGAagtacgaaga  TACTACTACTACGGTATGGACGTC   D6-13   JH5   60  
10  TGTGCGAGAGA tcagTGGCTGCCTATTAat   TACTTTGACTAC   D3-9   JH4   60  
11  GCGAGAG ggACGGTTCGGGGAGTTATTgggttatacttggactactacggtatg   GACGTC   D3-10   JH6   60  
12   TGTGCGAGAG cTAGTGGGAG CTATGACTAC   D1-26   JH4   60  
13   GCAAGAGA ccgGTATTACGATTTTTGGAGTGGTTATTATgtac   TTGACTAC   D3-3   JH4   65  
14   GCGAGA  cagcagttcgccccaTATTACGATTTTTGGAGTGGTTcctcttttcgatt   CTACTACTACGGTA   D3-3   JH6   60  
15   GCGAGAG   tcgcgggagtcGCATATTGTGGTGGTGACTGCTATTggagagagt   ACTACTTTGACTAC   D2-21   JH4   60  
16   GCGAGA  gatccccGCCATAGCAGCAGCTGGTACccctactactactacggtatg   GACGCT   D6-13   JH6   60  
17   GCGAACG   gTGTCAGGGGTGACTACgtgtatccg TCCGGTATGGACGTC   D4-17   JH6   60  
18  GCGAGACA tcgactAGGATATTGTAGTAGTACCAGCTGC   TACTACTACTACTACGGTA   D2-2   JH6   60  
19   GCGAAAGA   ttcggggtatcaGTATTACGATTTTTGGAGTGGTTATTATAacgata   ATTACTACTACTACTACGGTA   D3-3   JH6   60  
20   GCGAG gaaggagtcTTACGATTTTTGGAGTGGTTATTtgtcccgt   TACTACTACTACGGTA   D3-3   JH6   60  
21   GCGAGA actttgagaattccGTATTACTATGATAGTAGTGGTTATTATTggggct   ACAAC   D3-22   JH5   60  
22   GCG   agacggggGGGTATTGTAGTGGTGGTAGCTGCCATT TGGGACTAC   D2-15   JH4   60  
23   GCG   agatggtctttcggtTATTGTGCTGGTGGCATCTGttacggtggaaactaccaatactacgctatg   GACGTC   D2-15   JH6   60  
24   GCGAG   ccgcGATATTGTAGTGGTGGTAGCTGCTACCCCgg   AATACTACTACTACATGGACGTC   D2-15   JH6   66  
25  TGTATTACTGTGC cgtaTGTGGTGGTTATTACTATGC   TGACTAC   D2-21   JH4   60  
26  CTGTGCGAAAGA tagggatgACTATGGTaattacgt   CTTTGACTAC   D3-10   JH4   60  
27   GTGAGAGA  tacctccaGTATAGTAGGAGGTGGTCCccttgaccgt   TGG   D6-13   JH5   60  
28   GCAAGA   gatgcttggcggcccgctcgccccgcGTATTATGATTACGTTTGGGGGAGTTATCGCCAcggccccca   TGATGCTTTTGATATC   D3-16   JH3   60  
29   ACCACAG   cccgtcggtgcttgcgccccCGATATTTTGACTGGTTATTGGACCcgggggta   TGCTACTACGGTATGGACGTC   D3-9   JH6   60  
30   GCGAGA   gattcccacttGTATTACGATATTTTGACTGGTTATTCTActgaaatggag  GACTAC   D3-9   JH4   60  
31   GCGAGAGA tcgccccggGGGTATTGTAGTAGTACCAGCTGCTATGtgctactacggta   TGGACGTC   D2-2   JH6   60  
32   GCGGCCG cTGCAGATTTTTGGAGTGGTTCTa   AAGAC   D3-3   JH4   60  
33  TGCGAGTG gTTACTATGATTCGGGGAc   CTACTACGGTGTGGACGTC   D3-10   JH6   60  
34   GCAAAAGAT   tctgttcagatgAGCAACCCCTGGTACgaaggaggggg   CTTCTGGTTCGACCCC   D6-13   JH5   60  
35   GCGAGAAA   accaagtTATTACTATGATAGTAGTGGTTATTACTACtgga   ACTACTACTACTACGGTATGGACGTC  D3-22   JH6   NF  
36
 		 GCGAAAG
 		 gtgctagTTACTTTGGTTCGGGGAGTTTTTATAACgagcc
 		CTCTGACCACTGG
 		 D3-10
 		 JH4
 		 NF
 

Underlined sequences represent allele-specific oligonucleotides. The lower-case letters indicate the inserted nucleotides.

VH indicates variable segment of the immunoglobulin heavy chain gene; N, nucleotides; DH, diversity segment; JH, joining segment; Taa, annealing temperature; and NF, not found.

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