Analysis of GATA-1-induced erythromegakaryocytic maturation in 5 randomly selected G1ME clones derived from E13.5 Gata1- chimeric fetal livers
Clone No. . | Ter119 positive, % . | . | GPIb positive, % . | . | ||
|---|---|---|---|---|---|---|
| . | MIGR1 . | GATA-1 . | MIGR1 . | GATA-1 . | ||
| 1 | 8 | 18 | 31 | 72 | ||
| 2 | 10 | 31 | 20 | 63 | ||
| 3 | 5 | 22 | 14 | 70 | ||
| 4 | 8 | 30 | 18 | 50 | ||
| 5 | 11 | 37 | 33 | 77 | ||
Clone No. . | Ter119 positive, % . | . | GPIb positive, % . | . | ||
|---|---|---|---|---|---|---|
| . | MIGR1 . | GATA-1 . | MIGR1 . | GATA-1 . | ||
| 1 | 8 | 18 | 31 | 72 | ||
| 2 | 10 | 31 | 20 | 63 | ||
| 3 | 5 | 22 | 14 | 70 | ||
| 4 | 8 | 30 | 18 | 50 | ||
| 5 | 11 | 37 | 33 | 77 | ||
G1ME cells derived from E13.5 fetal livers were cultured in the same conditions that were used to derive G1ME cells from Gata1– ES cells, as described in Figure 1. Fetal liver G1ME clones were isolated by limiting dilution, expanded, and then infected with GATA-1 retrovirus or MIGR1 control. Ter119 and GPIb expressions were assessed by flow cytometry, as in Figures 3 and 7.