Analysis of GATA-1-induced erythromegakaryocytic maturation in 10 randomly selected G1ME-cell clones derived from Gata1- ES-cell differentiation
Clone no. . | Ter119 positive, % . | . | GPIb positive, % . | . | ||
|---|---|---|---|---|---|---|
| . | MIGR1 . | GATA-1 . | MIGR1 . | GATA-1 . | ||
| 1 | 3 | 24 | 13 | 61 | ||
| 2 | 5 | 17 | 18 | 71 | ||
| 3 | 4 | 18 | 7 | 56 | ||
| 4 | 1 | 19 | 11 | 55 | ||
| 5 | 4 | 19 | 13 | 77 | ||
| 6 | 3 | 15 | 8 | 75 | ||
| 7 | 5 | 18 | 15 | 77 | ||
| 8 | 2 | 17 | 11 | 63 | ||
| 9 | 1 | 10 | 7 | 62 | ||
| 10 | 3 | 19 | 10 | 61 | ||
Clone no. . | Ter119 positive, % . | . | GPIb positive, % . | . | ||
|---|---|---|---|---|---|---|
| . | MIGR1 . | GATA-1 . | MIGR1 . | GATA-1 . | ||
| 1 | 3 | 24 | 13 | 61 | ||
| 2 | 5 | 17 | 18 | 71 | ||
| 3 | 4 | 18 | 7 | 56 | ||
| 4 | 1 | 19 | 11 | 55 | ||
| 5 | 4 | 19 | 13 | 77 | ||
| 6 | 3 | 15 | 8 | 75 | ||
| 7 | 5 | 18 | 15 | 77 | ||
| 8 | 2 | 17 | 11 | 63 | ||
| 9 | 1 | 10 | 7 | 62 | ||
| 10 | 3 | 19 | 10 | 61 | ||
G1ME cells were maintained in liquid culture with Tpo. Individual clones were isolated by limiting dilution, expanded, and then infected with GATA-1 retrovirus or MIGR1 control. Ter119 and GPIb expression was assessed by flow cytometry, as described in Figures 3 and 7.