Sequences of real-time PCR primers
Primer . | Sequence, 5′-3′ . |
|---|---|
| FcRH5, forward | GGTGCATATCGCTGTACTGGA |
| FcRH5, reverse | AATGGCTCTTGGACTTGGATTTTGAC |
| EBNA2, forward | GGTTGCTGGAGAGGGCAAGG |
| EBNA2, reverse | GCCCAGAGGCTCCCATTCTC |
| 18S rRNA, forward | CACGGCCGGTACAGTGAAAC |
| 18S rRNA, reverse | CCCGTCGGCATGTATTAGCT |
| CD21, forward | TGTCTTCGGGAAAATGGAGT |
| CD21, reverse | ACCTTCCCATTGGGAAATCGT |
| FcRH5, ChIP no. 1, forward | GGGACAGGAATTGACAAGGT |
| FcRH5, ChIP no. 1, reverse | TCCTCATGCCAATACCACAC |
| FcRH5, ChIP no. 2, forward | TCTAGCAGAACGGCCAGATT |
| FcRH5, ChIP no. 2, reverse | GAGTGGAAGTCCTGGCTCAT |
| FcRH5, ChIP no. 3, forward | CGCATATGCCCCAATAAATG |
| FcRH5, ChIP no. 3, reverse | GCCCATACCTTGGTCTTCCT |
| FcRH5, ChIP no. 4, forward | ACTCCTCGTGGCAGCTAATG |
| FcRH5, ChIP no. 4, reverse | TTCCTGGAAAGTACACACAAAAA |
| FcRH5, ChIP no. 5, forward | TCTTCCCTTCTTGGCATGTT |
| FcRH5, ChIP no. 5, reverse | TCACAGTTTCCTACCCGATG |
| FcRH5, ChIP no. 6, forward | ACGGGTACTCCCACTTCCTT |
| FcRH5, ChIP no. 6, reverse | CACAGCAGCATGAAGACCTG |
| CD23, ChIP, forward | AACTCCAGGCCGTCCTTCTA |
| CD23, ChIP, reverse | ACCTAAATATGGGCTTGCCA |
Primer . | Sequence, 5′-3′ . |
|---|---|
| FcRH5, forward | GGTGCATATCGCTGTACTGGA |
| FcRH5, reverse | AATGGCTCTTGGACTTGGATTTTGAC |
| EBNA2, forward | GGTTGCTGGAGAGGGCAAGG |
| EBNA2, reverse | GCCCAGAGGCTCCCATTCTC |
| 18S rRNA, forward | CACGGCCGGTACAGTGAAAC |
| 18S rRNA, reverse | CCCGTCGGCATGTATTAGCT |
| CD21, forward | TGTCTTCGGGAAAATGGAGT |
| CD21, reverse | ACCTTCCCATTGGGAAATCGT |
| FcRH5, ChIP no. 1, forward | GGGACAGGAATTGACAAGGT |
| FcRH5, ChIP no. 1, reverse | TCCTCATGCCAATACCACAC |
| FcRH5, ChIP no. 2, forward | TCTAGCAGAACGGCCAGATT |
| FcRH5, ChIP no. 2, reverse | GAGTGGAAGTCCTGGCTCAT |
| FcRH5, ChIP no. 3, forward | CGCATATGCCCCAATAAATG |
| FcRH5, ChIP no. 3, reverse | GCCCATACCTTGGTCTTCCT |
| FcRH5, ChIP no. 4, forward | ACTCCTCGTGGCAGCTAATG |
| FcRH5, ChIP no. 4, reverse | TTCCTGGAAAGTACACACAAAAA |
| FcRH5, ChIP no. 5, forward | TCTTCCCTTCTTGGCATGTT |
| FcRH5, ChIP no. 5, reverse | TCACAGTTTCCTACCCGATG |
| FcRH5, ChIP no. 6, forward | ACGGGTACTCCCACTTCCTT |
| FcRH5, ChIP no. 6, reverse | CACAGCAGCATGAAGACCTG |
| CD23, ChIP, forward | AACTCCAGGCCGTCCTTCTA |
| CD23, ChIP, reverse | ACCTAAATATGGGCTTGCCA |
ChIP indicates chromatin immunoprecipitation.