S-phase suicide assay: SRC analysis
. | FACS analysis . | . | . | CFC assay % GFP§ . | ||
|---|---|---|---|---|---|---|
. | %hCD45* . | % GFP† . | P‡ . | . | ||
| -cyt | 28 ± 14 | 54 ± 20 | .029∥ | 39 ± 14 | ||
| +cyt | 42 ± 32 | 91 ± 5 | 68 ± 15 | |||
| -cyt Ara-C | 23 ± 22 | 29 ± 8 | .028¶ | 18 ± 13 | ||
| +cyt Ara-C | 7 ± 6 | 61 ± 22 | 45 ± 22 | |||
. | FACS analysis . | . | . | CFC assay % GFP§ . | ||
|---|---|---|---|---|---|---|
. | %hCD45* . | % GFP† . | P‡ . | . | ||
| -cyt | 28 ± 14 | 54 ± 20 | .029∥ | 39 ± 14 | ||
| +cyt | 42 ± 32 | 91 ± 5 | 68 ± 15 | |||
| -cyt Ara-C | 23 ± 22 | 29 ± 8 | .028¶ | 18 ± 13 | ||
| +cyt Ara-C | 7 ± 6 | 61 ± 22 | 45 ± 22 | |||
All values are expressed as mean ± SD of 2 experiments performed. For -cyt group, n = 4; for +cyt group, n = 4; for -cyt Ara-C group, n = 6; and for +cyt Ara-C group, n = 5.
n indicates number of mice per group.
Percentage of human cells in the mouse BM.
Percentage of human GFP-expressing cells.
P value calculated by rank sum 2-sided test.
Percentage of human GFP-expressing cells, scored by fluorescence microscopy.
-cyt versus +cyt.
-cyt Ara-C versus +cyt Ara-C.