S-phase suicide assay: CFC analysis
. | -cyt . | +cyt . | -cyt Ara-C . | +cyt Ara-C . |
|---|---|---|---|---|
| % GFP | 39 | 65 | 34 | 55 |
| CFCs | 80 | 83 | 40 | 16 |
| % killing | — | — | 50 | 80 |
. | -cyt . | +cyt . | -cyt Ara-C . | +cyt Ara-C . |
|---|---|---|---|---|
| % GFP | 39 | 65 | 34 | 55 |
| CFCs | 80 | 83 | 40 | 16 |
| % killing | — | — | 50 | 80 |
Human CD34+ were transduced in the presence (+cyt) or absence (-cyt) of cytokines for 18 hours, then exposed or not to Ara-C for another 12 hours, as indicated, plated in methylcellulose, and analyzed after 2 weeks. Average values of 2 experiments are shown.
% GFP indicates percentage of GFP+ colonies scored by fluorescence microscopy; CFCs, total number expressed as number of colonies per 103 CD34+ cells plated; % killing, percentage of killing calculated as the ratio between the number of colonies arising form the Ara-C—treated and —untreated populations.