S-phase suicide assay: HSPC cell-cycle analysis
Phase . | -cyt, % . | +cyt, % . | -cyt Ara-C, % . | +cyt Ara-C, % . |
|---|---|---|---|---|
| Apoptotic/necrotic cells; M1 | 32 | 28 | 42 | 42 |
| G0/G1; M2 | 52 | 43 | 48 | 49 |
| S; M3 | 9 | 17 | 6 | 5 |
| G2/M; M4 | 7 | 12 | 4 | 4 |
Phase . | -cyt, % . | +cyt, % . | -cyt Ara-C, % . | +cyt Ara-C, % . |
|---|---|---|---|---|
| Apoptotic/necrotic cells; M1 | 32 | 28 | 42 | 42 |
| G0/G1; M2 | 52 | 43 | 48 | 49 |
| S; M3 | 9 | 17 | 6 | 5 |
| G2/M; M4 | 7 | 12 | 4 | 4 |
Human CD34+ were stimulated (+cyt) or not (-cyt) with cytokines for 18 hours, then exposed or not to Ara-C for another 12 hours, as indicated, and analyzed immediately. Distribution of the analyzed cell populations is shown in the indicated cell-cycle phases, marked as M1 to M4 in Figure 2A. Average values of 2 experiments are shown.