Increased numbers of HSC cells in the BM and spleen of SHIP-/- mice compared with WT littermates
. | Increase in WBM cells, % . | . | . | Absolute no. cells per pair of femur and tiba . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| Population . | SHIP-/- . | WT . | P . | SHIP-/- . | WT . | P . | ||||
| Lin-Sca1+cKit+Thy1low | ||||||||||
| BM | 0.4114 ± 0.0353 | 0.0725 ± 0.025 | < .001 | (20.53 ± 3.39) × 104 | (3.79 ± 1.53) × 104 | .001 | ||||
| Spleen | 0.0957 ± 0.0131 | 0.0142 ± 0.0040 | .004 | (50.90 ± 9.25) × 103 | (5.80 ± 1.92) × 103 | .009 | ||||
| Lin-Sca1+cKit+Flk2- | ||||||||||
| BM | 0.2860 ± 0.0398 | 0.1340 ± 0.0202 | .009 | (22.14 ± 4.59) × 104 | (10.3 ± 7.22) × 104 | .034 | ||||
| Spleen | 0.0827 ± 0.0141 | 0.027 ± 0.002 | .017 | (52.10 ± 7.89) × 103 | (13.58 ± 0.75) × 103 | .008 | ||||
| Lin-Sca1+cKit+CD48- | ||||||||||
| BM | 0.019 ± 0.002 | 0.0068 ± 0.0006 | .001 | (7.84 ± 0.95) × 103 | (2.76 ± 0.30) × 103 | < .001 | ||||
| Spleen | 0.0020 ± 0.0003 | 0.00023 ± 0.00005 | .005 | (55.3 ± 0.73) × 102 | (2.89 ± 0.87) × 102 | .002 | ||||
| Lin-Sca1+cKit+CD48-CD150+ | ||||||||||
| BM | 0.00084 ± 0.00001 | 0.0004 ± 0.0001 | .039 | (3.5 ± 0.19) × 102 | (1.64 ± 0.62) × 102 | .045 | ||||
| Side population | ||||||||||
| BM | 0.0565 ± 0.0044 | 0.0290 ± 0.0044 | .005 | (26.7 ± 4.7) × 103 | (12.5 ± 2.4) × 103 | .035 | ||||
. | Increase in WBM cells, % . | . | . | Absolute no. cells per pair of femur and tiba . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| Population . | SHIP-/- . | WT . | P . | SHIP-/- . | WT . | P . | ||||
| Lin-Sca1+cKit+Thy1low | ||||||||||
| BM | 0.4114 ± 0.0353 | 0.0725 ± 0.025 | < .001 | (20.53 ± 3.39) × 104 | (3.79 ± 1.53) × 104 | .001 | ||||
| Spleen | 0.0957 ± 0.0131 | 0.0142 ± 0.0040 | .004 | (50.90 ± 9.25) × 103 | (5.80 ± 1.92) × 103 | .009 | ||||
| Lin-Sca1+cKit+Flk2- | ||||||||||
| BM | 0.2860 ± 0.0398 | 0.1340 ± 0.0202 | .009 | (22.14 ± 4.59) × 104 | (10.3 ± 7.22) × 104 | .034 | ||||
| Spleen | 0.0827 ± 0.0141 | 0.027 ± 0.002 | .017 | (52.10 ± 7.89) × 103 | (13.58 ± 0.75) × 103 | .008 | ||||
| Lin-Sca1+cKit+CD48- | ||||||||||
| BM | 0.019 ± 0.002 | 0.0068 ± 0.0006 | .001 | (7.84 ± 0.95) × 103 | (2.76 ± 0.30) × 103 | < .001 | ||||
| Spleen | 0.0020 ± 0.0003 | 0.00023 ± 0.00005 | .005 | (55.3 ± 0.73) × 102 | (2.89 ± 0.87) × 102 | .002 | ||||
| Lin-Sca1+cKit+CD48-CD150+ | ||||||||||
| BM | 0.00084 ± 0.00001 | 0.0004 ± 0.0001 | .039 | (3.5 ± 0.19) × 102 | (1.64 ± 0.62) × 102 | .045 | ||||
| Side population | ||||||||||
| BM | 0.0565 ± 0.0044 | 0.0290 ± 0.0044 | .005 | (26.7 ± 4.7) × 103 | (12.5 ± 2.4) × 103 | .035 | ||||
At least 3 mice were tested for each genotype.