Lymphocyte counts in the organs of RAG-1-deficient mice, RAG1-transduced mice, control mice, and wild-type C57BL/10 mice 6 months after injection of ex vivo-transduced Sca-1 cells
. | Thymus, × 106 cells . | Lymph nodes, × 106 cells . | Spleen, × 106 cells . | Bone marrow, × 106 cells . |
|---|---|---|---|---|
| RAG-1–/–; n = 4 | 0.2 ± 0.1 | 3 ± 2 | 4 ± 1 | 35 ± 11 |
| RAG1-TM, 3 Gy; n = 5 | 3 ± 4 | 15 ± 3 | 35 ± 8 | 36 ± 11 |
| RAG1-TM, 8 Gy; n = 4 | 11 ± 4 | 14 ± 5 | 33 ± 14 | 34 ± 3 |
| WT-CTRL; n = 8 | 14 ± 5 | 18 ± 6 | 28 ± 6 | 30 ± 5 |
| C56BL/10; n = 4 | 75 ± 27 | 15 ± 6 | 36 ± 13 | 45 ± 12 |
. | Thymus, × 106 cells . | Lymph nodes, × 106 cells . | Spleen, × 106 cells . | Bone marrow, × 106 cells . |
|---|---|---|---|---|
| RAG-1–/–; n = 4 | 0.2 ± 0.1 | 3 ± 2 | 4 ± 1 | 35 ± 11 |
| RAG1-TM, 3 Gy; n = 5 | 3 ± 4 | 15 ± 3 | 35 ± 8 | 36 ± 11 |
| RAG1-TM, 8 Gy; n = 4 | 11 ± 4 | 14 ± 5 | 33 ± 14 | 34 ± 3 |
| WT-CTRL; n = 8 | 14 ± 5 | 18 ± 6 | 28 ± 6 | 30 ± 5 |
| C56BL/10; n = 4 | 75 ± 27 | 15 ± 6 | 36 ± 13 | 45 ± 12 |
Data are expressed as mean ± SD. The Mann-Whitney U test has been used to compare the lymphocyte counts in each group. After gene therapy, the comparison between the thymocyte counts in the 3 Gy- and 8 Gy-irradiated recipient is statistically significant (P < .05). No significant difference was observed in lymph nodes, spleen, and bone marrow cell counts.
RAG-1–/– indicates RAG-1-deficient mice; RAG1-TM, RAG1-transduced mice; and WT-CTRL, control mice.