Expression of IGnT transcripts in human reticulocyte-enriched fraction was analyzed by real-time PCR. Standard curves for IGnTs and GAPDH were generated by serial dilution of each plasmid DNA. The expression level ofIGnT transcript was normalized to that of GAPDHtranscript, measured in the same cDNAs. Data were obtained from triplicate experiments and are expressed as mean ± SD.

ND indicates not detected.