Killer cell receptor and effector cell marker transcripts in single CD8+CD94+ T cells expressing the same TCR as the respective dominant clones in LGL 1-5 and BD 2-3
| . | LGL 1 . | LGL 2 . | LGL 3 . | LGL 4 . | LGL 5 . | BD 1 . | BD 2(1)* . | BD 2(2)† . |
|---|---|---|---|---|---|---|---|---|
| T-cell receptor‡ . | BV17 . | BV9 . | BV13 . | BV12 . | BV8 . | BV7 . | BV3 . | BV3 . |
| (163)1-153 . | (154) . | (176) . | (196) . | (164) . | (227) . | (197) . | (201) . | |
| Killer cell receptors | ||||||||
| NKG2A | 0/101-155 | 0/10 | 0/10 | 0/10 | 3/10 | 0/10 | 0/5 | 0/6 |
| NKG2A 101-154 | − | − | − | − | + | − | − | − |
| NKG2C/E/F# | 5/10 | 2/10 | 5/10 | 0/10 | 1/10 | 4/10 | 2/5 | 3/6 |
| NKG2C/E/F 101-154 | + | + | + | − | + | + | + | + |
| NKG2D | 6/10 | 4/10 | 4/10 | 2/10 | 1/10 | 3/10 | 0/5 | 3/6 |
| KIRL | 0/10 | 0/10 | 6/10 | 0/10 | 7/10 | 2/10 | 0/5 | 3/6 |
| KIRL 101-154 | − | − | + | − | + | + | + | + |
| KIRS | 0/10 | 0/10 | 4/10 | 0/10 | 1/10 | 4/10 | 0/5 | 3/6 |
| KIRS 101-154 | − | − | + | − | + | + | + | + |
| Effector cell markers | ||||||||
| Granz B | 10/10 | 8/10 | 10/10 | 6/10 | 7/10 | 9/10 | 4/5 | 5/6 |
| IFNγ | 7/10 | 5/10 | 7/10 | 4/10 | 0/10 | 4/10 | 0/5 | 0/6 |
| TNFα | 4/10 | 6/10 | 5/10 | 3/10 | 6/10 | 4/10 | 3/5 | 4/6 |
| FasL | 1/10 | 2/10 | 4/10 | 1/10 | 2/10 | 2/10 | 1/5 | 0/6 |
| Controls | ||||||||
| CD3 | 6/10 | 9/10 | 8/10 | 8/10 | 9/10 | 10/10 | 3/5 | 3/6 |
| CD94 | 9/10 | 6/10 | 5/10 | 7/10 | 3/10 | 7/10 | 1/5 | 1/6 |
| . | LGL 1 . | LGL 2 . | LGL 3 . | LGL 4 . | LGL 5 . | BD 1 . | BD 2(1)* . | BD 2(2)† . |
|---|---|---|---|---|---|---|---|---|
| T-cell receptor‡ . | BV17 . | BV9 . | BV13 . | BV12 . | BV8 . | BV7 . | BV3 . | BV3 . |
| (163)1-153 . | (154) . | (176) . | (196) . | (164) . | (227) . | (197) . | (201) . | |
| Killer cell receptors | ||||||||
| NKG2A | 0/101-155 | 0/10 | 0/10 | 0/10 | 3/10 | 0/10 | 0/5 | 0/6 |
| NKG2A 101-154 | − | − | − | − | + | − | − | − |
| NKG2C/E/F# | 5/10 | 2/10 | 5/10 | 0/10 | 1/10 | 4/10 | 2/5 | 3/6 |
| NKG2C/E/F 101-154 | + | + | + | − | + | + | + | + |
| NKG2D | 6/10 | 4/10 | 4/10 | 2/10 | 1/10 | 3/10 | 0/5 | 3/6 |
| KIRL | 0/10 | 0/10 | 6/10 | 0/10 | 7/10 | 2/10 | 0/5 | 3/6 |
| KIRL 101-154 | − | − | + | − | + | + | + | + |
| KIRS | 0/10 | 0/10 | 4/10 | 0/10 | 1/10 | 4/10 | 0/5 | 3/6 |
| KIRS 101-154 | − | − | + | − | + | + | + | + |
| Effector cell markers | ||||||||
| Granz B | 10/10 | 8/10 | 10/10 | 6/10 | 7/10 | 9/10 | 4/5 | 5/6 |
| IFNγ | 7/10 | 5/10 | 7/10 | 4/10 | 0/10 | 4/10 | 0/5 | 0/6 |
| TNFα | 4/10 | 6/10 | 5/10 | 3/10 | 6/10 | 4/10 | 3/5 | 4/6 |
| FasL | 1/10 | 2/10 | 4/10 | 1/10 | 2/10 | 2/10 | 1/5 | 0/6 |
| Controls | ||||||||
| CD3 | 6/10 | 9/10 | 8/10 | 8/10 | 9/10 | 10/10 | 3/5 | 3/6 |
| CD94 | 9/10 | 6/10 | 5/10 | 7/10 | 3/10 | 7/10 | 1/5 | 1/6 |
Clone 1 of BD 2 (TCR: BV3BJ2.5).
Clone 2 of BD 2 (TCR: BV3BJ2.7).
Only cDNAs from single cells sorted with windows of Figure 1containing the transcript of the LGL-TCR have been analyzed.
Characteristic migration by Genescan.
Number of positive cells per number of cells tested.
Presence (+) or absence (−) of the respective transcript in the cDNA prepared from 10 cells sorted with windows of Figure 1.
#PCR with primers for the 3 activating NKG2 molecules.