Effects of p38 and ERK1/2 MAP kinase inhibition on HMGB1-stimulated cytokine release from HMEC-1 cells
| . | IL-8 (pg/mL) . | TNFα (pg/mL) . |
|---|---|---|
| p38 | ||
| Media | 37.5 ± 16.9 | 0.61 ± 0.4 |
| DMSO | 33.5 ± 15.2 | 2.8 ± 1.2 |
| SB203580 | 16.2 ± 8.1 | 6.1 ± 3.1 |
| HMGB1 | 2296.8 ± 140.2 | 502.7 ± 91.7 |
| DMSO + HMGB1 | 2165.9 ± 257.3 | 463.9 ± 76.3 |
| SB203580 + HMGB1 | 785.7 ± 63.2* | 444.4 ± 88.7 |
| ERK1 | ||
| Media | 57.2 ± 12.9 | 0.67 ± 0.4 |
| DMSO | 59.6 ± 11.3 | 0.99 ± 0.1 |
| PD90859 | 54.2 ± 14.3 | 0.82 ± 0.5 |
| HMGB1 | 1824.8 ± 465.2 | 228.1 ± 26.9 |
| DMSO + HMGB1 | 1850 ± 422.6 | 244.5 ± 36.7 |
| PD90859 + HMGB1 | 1843.2 ± 348.4 | 208 ± 42.4 |
| ERK2 | ||
| Media | 5.2 ± 0.2 | 5.7 ± 0.02 |
| DMSO | 4.1 ± 0.1 | 5.8 ± 0.2 |
| SB203580 + PD90859 | 2.8 ± 0.1 | 5.7 ± 0.1 |
| HMGB1 | 924.0 ± 181.0 | 292.4 ± 81.2 |
| DMSO + HMGB1 | 982.4 ± 22.5 | 223 ± 13.5 |
| SB203580 + PD90859 + HMGB1 | 127.6 ± 7.36† | 183.6 ± 11.9 |
| . | IL-8 (pg/mL) . | TNFα (pg/mL) . |
|---|---|---|
| p38 | ||
| Media | 37.5 ± 16.9 | 0.61 ± 0.4 |
| DMSO | 33.5 ± 15.2 | 2.8 ± 1.2 |
| SB203580 | 16.2 ± 8.1 | 6.1 ± 3.1 |
| HMGB1 | 2296.8 ± 140.2 | 502.7 ± 91.7 |
| DMSO + HMGB1 | 2165.9 ± 257.3 | 463.9 ± 76.3 |
| SB203580 + HMGB1 | 785.7 ± 63.2* | 444.4 ± 88.7 |
| ERK1 | ||
| Media | 57.2 ± 12.9 | 0.67 ± 0.4 |
| DMSO | 59.6 ± 11.3 | 0.99 ± 0.1 |
| PD90859 | 54.2 ± 14.3 | 0.82 ± 0.5 |
| HMGB1 | 1824.8 ± 465.2 | 228.1 ± 26.9 |
| DMSO + HMGB1 | 1850 ± 422.6 | 244.5 ± 36.7 |
| PD90859 + HMGB1 | 1843.2 ± 348.4 | 208 ± 42.4 |
| ERK2 | ||
| Media | 5.2 ± 0.2 | 5.7 ± 0.02 |
| DMSO | 4.1 ± 0.1 | 5.8 ± 0.2 |
| SB203580 + PD90859 | 2.8 ± 0.1 | 5.7 ± 0.1 |
| HMGB1 | 924.0 ± 181.0 | 292.4 ± 81.2 |
| DMSO + HMGB1 | 982.4 ± 22.5 | 223 ± 13.5 |
| SB203580 + PD90859 + HMGB1 | 127.6 ± 7.36† | 183.6 ± 11.9 |