Effects of rhHMGB1 on human lung microvascular endothelial cells
| Response . | Condition after 24-hour incubation . | ||
|---|---|---|---|
| Media . | rhHMGB1 (50 ng/mL) . | TNFα (10 ng/mL) . | |
| ICAM-1 (abs × 103/cell) | 3.17 ± 0.32 | 41.32 ± 8.12* | 206.29 ± 81.31 |
| VCAM-1 (abs × 103/cell) | 7.46 ± 0.53 | 145.35 ± 9.08* | 584.16 ± 28.8 |
| TNFα (pg/mL) | 0 | 1238 ± 236* | 11 634 ± 3258 |
| IL-8 (ng/mL) | 0.83 ± 0.36 | 9.09 ± 1.20* | 89.23 ± 26.00 |
| MCP-1 (ng/mL) | 3.54 ± 1.53 | 10.94 ± 2.10* | 85.75 ± 13.71 |
| PAI-1 (ng/mL) | 1024 ± 276 | 2614 ± 552* | 3478 ± 552 |
| tPA (ng/mL) | 5.55 ± 0.85 | 6.71 ± 0.20 | 9.51 ± 0.32 |
| Response . | Condition after 24-hour incubation . | ||
|---|---|---|---|
| Media . | rhHMGB1 (50 ng/mL) . | TNFα (10 ng/mL) . | |
| ICAM-1 (abs × 103/cell) | 3.17 ± 0.32 | 41.32 ± 8.12* | 206.29 ± 81.31 |
| VCAM-1 (abs × 103/cell) | 7.46 ± 0.53 | 145.35 ± 9.08* | 584.16 ± 28.8 |
| TNFα (pg/mL) | 0 | 1238 ± 236* | 11 634 ± 3258 |
| IL-8 (ng/mL) | 0.83 ± 0.36 | 9.09 ± 1.20* | 89.23 ± 26.00 |
| MCP-1 (ng/mL) | 3.54 ± 1.53 | 10.94 ± 2.10* | 85.75 ± 13.71 |
| PAI-1 (ng/mL) | 1024 ± 276 | 2614 ± 552* | 3478 ± 552 |
| tPA (ng/mL) | 5.55 ± 0.85 | 6.71 ± 0.20 | 9.51 ± 0.32 |
Mean ± SEM of 3 experiments; abs indicates antibody binding sites/cell.
P < .05 rhHMGB1 compared with media alone.