Table 2. MHC class II molecules acquired... | American Society of Hematology

Table 2.

MHC class II molecules acquired by T cells are stable in culture

H2-A^k expression on 3A9 cells isolated from 3A9-HEL_46-61 pulsed DAP3 H2-A^k coculture
Time after sorting, h	MFI	% in M1 gate
0	23.46	27.05
3	17.14	32.86
18	11.99	17.07

H2-A^k expression on 3A9 cells isolated from 3A9-HEL_46-61 pulsed DAP3 H2-A^k coculture
Time after sorting, h	MFI	% in M1 gate
0	23.46	27.05
3	17.14	32.86
18	11.99	17.07

3A9 cells were cocultured with CFSE-labeled DAP.3 H2-A^k pulsed with HEL_46-61 peptide. To isolate 3A9 cells from the coculture, 3A9 cells, which were CFSE negative, were sorted. The sorted 3A9 cells, devoid of any APCs, then were cultured in medium. The sorted 3A9 cells were stained simultaneously with cy-chrome–conjugated anti-CD4 and PE-conjugated anti–H2-A^k at different time intervals. The level of H2-A^k on 3A9 was analysis by flow cytometry. Only the CD4⁺ population was gated for analysis. 3A9 control: MFI = 5.15; % in M1 gate = 0.09.

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