Stimulation of AML G0 cells into active cell cycle
| Culture condition . | % of total cells in G06-150 . | ||
|---|---|---|---|
| Time 0 . | 24 h . | 72 h . | |
| Patient 1 | |||
| No GF | 99.8 ± 0 | 80.0 | 37.0 ± 4.2 |
| SF + FL + IL-3 | 66.7 | 0 ± 0 | |
| Patient 2 | |||
| No GF | 98.7 ± 1.0 | 22.4 | 16.8 ± 14.4 |
| SF + FL + IL-3 | 12.4 | 7.6 ± 0.5 | |
| Patient 4 | |||
| No GF | 84.4 ± 19.6 | 2.1 | 1.2 ± 0.1 |
| SF + FL + IL-3 | 1.1 | 0.6 ± 0.4 | |
| Patient 5 | |||
| No GF | 93.4 ± 6.8 | 3.1 | 11.9 ± 15.3 |
| SF + FL + IL-3 | 1.3 | 2.0 ± 2.0 | |
| Lin−NBM A, No GF | ND | 95.9 | 88.3 |
| Lin− NBM B, No GF | ND | 99.9 | 98.3 |
| Culture condition . | % of total cells in G06-150 . | ||
|---|---|---|---|
| Time 0 . | 24 h . | 72 h . | |
| Patient 1 | |||
| No GF | 99.8 ± 0 | 80.0 | 37.0 ± 4.2 |
| SF + FL + IL-3 | 66.7 | 0 ± 0 | |
| Patient 2 | |||
| No GF | 98.7 ± 1.0 | 22.4 | 16.8 ± 14.4 |
| SF + FL + IL-3 | 12.4 | 7.6 ± 0.5 | |
| Patient 4 | |||
| No GF | 84.4 ± 19.6 | 2.1 | 1.2 ± 0.1 |
| SF + FL + IL-3 | 1.1 | 0.6 ± 0.4 | |
| Patient 5 | |||
| No GF | 93.4 ± 6.8 | 3.1 | 11.9 ± 15.3 |
| SF + FL + IL-3 | 1.3 | 2.0 ± 2.0 | |
| Lin−NBM A, No GF | ND | 95.9 | 88.3 |
| Lin− NBM B, No GF | ND | 99.9 | 98.3 |
ND indicates not done.
The percentage of G0 cells at time 0 was determined by reanalyzing sorted G0 cells with Hst/PY staining. Each value at time 0 and 72 hours for AML samples is the mean ± SD result from 2 independent experiments while values at 24 hours for AML samples and at 24 and 72 hours for Lin− NBM G0 cells are from 1 experiment.