Addition of IVIGs to cultured B lymphocytes reduced the number of cycling cells
| Time, days . | Intracellular expression of Ki-67 . | |||
|---|---|---|---|---|
| Control . | IVIGs . | |||
| % . | MFI . | % . | MFI . | |
| 0 | 1.5 | 2.5 | 1.5 | 2.5 |
| 1 | 0.6 | 2.4 | 0.7 | 2.3 |
| 2 | 3.4 | 4.0 | 3.2 | 3.4 |
| 3 | 29.0 | 6.1 | 17.7 | 6.7 |
| 4 | 79.0 | 34.3 | 65.0 | 20.3 |
| 5 | 88.9 | 41.9 | 70.3 | 23.2 |
| Time, days . | Intracellular expression of Ki-67 . | |||
|---|---|---|---|---|
| Control . | IVIGs . | |||
| % . | MFI . | % . | MFI . | |
| 0 | 1.5 | 2.5 | 1.5 | 2.5 |
| 1 | 0.6 | 2.4 | 0.7 | 2.3 |
| 2 | 3.4 | 4.0 | 3.2 | 3.4 |
| 3 | 29.0 | 6.1 | 17.7 | 6.7 |
| 4 | 79.0 | 34.3 | 65.0 | 20.3 |
| 5 | 88.9 | 41.9 | 70.3 | 23.2 |
Purified human B lymphocytes were cultured with IL-4 and L4.5 cells in the presence of human serum albumin (9 mg/mL) and sucrose (15 mg/mL) or IVIGs (15 mg/mL). Expression of intracellular Ki-67 was analyzed in permeabilized B lymphocytes with anti-Ki-67-PE as described in “Materials and methods.” All flow cytometry analyses were done with the FACScalibur cytometer using Cell Quest software.