Table 1. CD4 T-cell responses in primary... | American Society of Hematology

Table 1.

CD4⁺ T-cell responses in primary culture

	Control T cells	G-CSF T cells	ProGP-1 T cells
Cpm	72.5 ± 4.6	55.8 ± 4.2	61.6 ± 4.8
IFNγ	31.1 ± 3.1	37.5 ± 3.5	35.7 ± 1.1
IL-4	523 ± 34	2064 ± 81^*	1221 ± 120^*
IL-10	383 ± 191	917 ± 72^*	935 ± 145^*

	Control T cells	G-CSF T cells	ProGP-1 T cells
Cpm	72.5 ± 4.6	55.8 ± 4.2	61.6 ± 4.8
IFNγ	31.1 ± 3.1	37.5 ± 3.5	35.7 ± 1.1
IL-4	523 ± 34	2064 ± 81^*	1221 ± 120^*
IL-10	383 ± 191	917 ± 72^*	935 ± 145^*

Naive B6 (H2^b) mice received control diluent, G-CSF, or ProGP-1 as described in “Materials and methods.” Splenic CD4⁺ T cells were purified by magnetic separation or FACS (as described in “Materials and methods”) and stimulated in primary culture by plate-bound antibodies to CD3 and CD28 (both at 10 μg/mL). Results represent mean ± SE of triplicate wells.

P < .05 versus control T cells. Proliferative responses (×10³) were measured by ³H incorporation. IFNγ (U/mL), IL-4 (pg/mL), and IL-10 (pg/mL) were determined in culture supernatants by ELISA.

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