PCR analysis of body fluids for vector sequences
| Sample . | Subject . | Baseline . | Days after injection . | Weeks after injection . | 24 . | |||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 0 . | 1 . | 2 . | 3 . | 4 . | 5 . | 6 . | 7 . | 2 . | 3 . | 4 . | 5 . | 6 . | 7 . | 8 . | 10 . | 12 . | 14 . | 16 . | ||||
| Serum | A6-150 | − | ND | + | + | ND | − | ND | ND | − | ND | ND | ND | ND | ND | ND | ND | ND | − | − | − | ND |
| B6-150 | − | + | + | − | ND | ND | ND | ND | − | − | − | − | ND | − | ND | − | − | ND | ND | ND | ND | |
| C6-150 | − | − | + | + | ND | ND | ND | − | − | ND | − | − | ND | − | ND | − | ND | ND | ND | ND | ND | |
| D | − | − | + | + | ND | ND | ND | ND | − | ND | − | − | − | − | − | − | ND | ND | ND | ND | ND | |
| E | − | ND | ND | + | ND | ND | ND | ND | + | − | ND | + | ND | ND | + | − | − | + | ND | ND | ND | |
| F | − | ND | + | ND | ND | ND | ND | ND | + | ND | ND | − | ND | − | ND | − | ND | − | ND | ND | ND | |
| G | − | − | + | + | ND | ND | ND | ND | + | − | − | ND | ND | ND | ND | ND | ND | − | ND | ND | ND | |
| H | − | ND | ND | ND | ND | + | ND | ND | + | − | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | |
| Saliva | A6-150 | − | − | + | − | ND | ND | ND | ND | − | ND | ND | − | ND | ND | − | − | ND | ND | ND | ND | ND |
| B6-150 | − | ND | + | − | − | ND | ND | ND | − | − | − | − | − | − | − | ND | ND | ND | ND | ND | ND | |
| C6-150 | − | − | + | − | ND | ND | ND | ND | − | ND | ND | − | ND | − | ND | ND | ND | ND | ND | ND | ND | |
| D | − | + | + | − | ND | ND | ND | ND | ND | − | − | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | |
| E | − | ND | ND | + | ND | ND | ND | ND | − | ND | ND | ND | ND | ND | − | − | − | − | ND | ND | ND | |
| F | − | ND | + | ND | ND | ND | ND | ND | − | ND | ND | − | ND | − | ND | − | ND | ND | ND | ND | ND | |
| G | − | fp | fp | + | ND | ND | ND | ND | − | + | − | ND | ND | ND | ND | ND | ND | − | ND | ND | ND | |
| H | − | ND | ND | ND | − | − | ND | ND | − | − | − | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | |
| Urine | A6-150 | − | − | − | − | ND | ND | ND | ND | ND | − | ND | − | ND | ND | − | − | ND | ND | ND | ND | ND |
| B6-150 | − | ND | + | − | ND | ND | ND | ND | − | − | − | − | − | − | − | ND | ND | ND | ND | ND | ND | |
| C6-150 | − | ND | − | − | ND | ND | ND | − | − | ND | − | − | ND | ND | ND | ND | ND | ND | ND | ND | ND | |
| D | − | − | − | − | ND | ND | ND | ND | − | − | − | ND | ND | ND | ND | − | ND | ND | ND | ND | ND | |
| E | − | ND | − | ND | ND | ND | ND | ND | − | − | − | − | ND | ND | − | − | − | − | ND | ND | ND | |
| F | − | + | ND | ND | ND | ND | ND | ND | − | ND | ND | − | ND | − | ND | − | ND | − | ND | ND | ND | |
| G | − | − | − | − | ND | ND | ND | ND | − | − | − | − | ND | ND | ND | ND | ND | − | ND | ND | ND | |
| H | − | ND | ND | ND | − | − | ND | ND | − | − | − | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | |
| Semen | A6-150 | − | ND | ND | − | − | ND | ND | ND | − | ND | ND | ND | ND | ND | − | ND | ND | ND | ND | ND | ND |
| B6-150 | − | ND | ND | ND | ND | − | ND | ND | − | ND | ND | − | − | − | − | ND | ND | ND | ND | ND | ND | |
| C6-150 | − | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | − | ND | ND | ND | ND | ND | |
| D | − | ND | ND | ND | − | ND | ND | ND | ND | − | − | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | |
| E | − | ND | ND | ND | ND | ND | ND | ND | − | ND | ND | − | ND | ND | ND | − | ND | ND | ND | ND | ND | |
| F | − | ND | ND | ND | ND | ND | ND | ND | − | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | − | |
| H | fp | ND | ND | ND | ND | − | ND | ND | − | − | − | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | |
| Sample . | Subject . | Baseline . | Days after injection . | Weeks after injection . | 24 . | |||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 0 . | 1 . | 2 . | 3 . | 4 . | 5 . | 6 . | 7 . | 2 . | 3 . | 4 . | 5 . | 6 . | 7 . | 8 . | 10 . | 12 . | 14 . | 16 . | ||||
| Serum | A6-150 | − | ND | + | + | ND | − | ND | ND | − | ND | ND | ND | ND | ND | ND | ND | ND | − | − | − | ND |
| B6-150 | − | + | + | − | ND | ND | ND | ND | − | − | − | − | ND | − | ND | − | − | ND | ND | ND | ND | |
| C6-150 | − | − | + | + | ND | ND | ND | − | − | ND | − | − | ND | − | ND | − | ND | ND | ND | ND | ND | |
| D | − | − | + | + | ND | ND | ND | ND | − | ND | − | − | − | − | − | − | ND | ND | ND | ND | ND | |
| E | − | ND | ND | + | ND | ND | ND | ND | + | − | ND | + | ND | ND | + | − | − | + | ND | ND | ND | |
| F | − | ND | + | ND | ND | ND | ND | ND | + | ND | ND | − | ND | − | ND | − | ND | − | ND | ND | ND | |
| G | − | − | + | + | ND | ND | ND | ND | + | − | − | ND | ND | ND | ND | ND | ND | − | ND | ND | ND | |
| H | − | ND | ND | ND | ND | + | ND | ND | + | − | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | |
| Saliva | A6-150 | − | − | + | − | ND | ND | ND | ND | − | ND | ND | − | ND | ND | − | − | ND | ND | ND | ND | ND |
| B6-150 | − | ND | + | − | − | ND | ND | ND | − | − | − | − | − | − | − | ND | ND | ND | ND | ND | ND | |
| C6-150 | − | − | + | − | ND | ND | ND | ND | − | ND | ND | − | ND | − | ND | ND | ND | ND | ND | ND | ND | |
| D | − | + | + | − | ND | ND | ND | ND | ND | − | − | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | |
| E | − | ND | ND | + | ND | ND | ND | ND | − | ND | ND | ND | ND | ND | − | − | − | − | ND | ND | ND | |
| F | − | ND | + | ND | ND | ND | ND | ND | − | ND | ND | − | ND | − | ND | − | ND | ND | ND | ND | ND | |
| G | − | fp | fp | + | ND | ND | ND | ND | − | + | − | ND | ND | ND | ND | ND | ND | − | ND | ND | ND | |
| H | − | ND | ND | ND | − | − | ND | ND | − | − | − | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | |
| Urine | A6-150 | − | − | − | − | ND | ND | ND | ND | ND | − | ND | − | ND | ND | − | − | ND | ND | ND | ND | ND |
| B6-150 | − | ND | + | − | ND | ND | ND | ND | − | − | − | − | − | − | − | ND | ND | ND | ND | ND | ND | |
| C6-150 | − | ND | − | − | ND | ND | ND | − | − | ND | − | − | ND | ND | ND | ND | ND | ND | ND | ND | ND | |
| D | − | − | − | − | ND | ND | ND | ND | − | − | − | ND | ND | ND | ND | − | ND | ND | ND | ND | ND | |
| E | − | ND | − | ND | ND | ND | ND | ND | − | − | − | − | ND | ND | − | − | − | − | ND | ND | ND | |
| F | − | + | ND | ND | ND | ND | ND | ND | − | ND | ND | − | ND | − | ND | − | ND | − | ND | ND | ND | |
| G | − | − | − | − | ND | ND | ND | ND | − | − | − | − | ND | ND | ND | ND | ND | − | ND | ND | ND | |
| H | − | ND | ND | ND | − | − | ND | ND | − | − | − | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | |
| Semen | A6-150 | − | ND | ND | − | − | ND | ND | ND | − | ND | ND | ND | ND | ND | − | ND | ND | ND | ND | ND | ND |
| B6-150 | − | ND | ND | ND | ND | − | ND | ND | − | ND | ND | − | − | − | − | ND | ND | ND | ND | ND | ND | |
| C6-150 | − | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | − | ND | ND | ND | ND | ND | |
| D | − | ND | ND | ND | − | ND | ND | ND | ND | − | − | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | |
| E | − | ND | ND | ND | ND | ND | ND | ND | − | ND | ND | − | ND | ND | ND | − | ND | ND | ND | ND | ND | |
| F | − | ND | ND | ND | ND | ND | ND | ND | − | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | − | |
| H | fp | ND | ND | ND | ND | − | ND | ND | − | − | − | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | |
No semen samples were taken from subject G due to erectile dysfunction secondary to sertraline. Semen analysis in subject H was faintly positive with nonspecific bands.
+indicates positive; −, negative; fp, faint positive; and ND, not done.
See Kay et al.24