Table 1. Oligonucleotide primers for... | American Society of Hematology

Table 1.

Oligonucleotide primers for amplification of coding region and 9 FECSs of JunB gene

	Forward primer (5′→3′)	Location	Reverse primer (5′→3′)	Location	Annealing temperature, °C
Coding region-1	cgacggccaatcgga	5639-5653	ggctcggtttcaggagtttg	6112-6193	60
Coding region-2	tggctgctacccgcc	5854-5868	gagacgcgccggtgt	6235-6221	62
Coding region-3	gctcggacaccggcg	6215-6229	gttggtgtaaacggg	6549-6528	62
Coding region-4	cccgtttacaccaactca	6535-6553	cttcaccttgtcctccagg	6900-6882	64
Coding region-5	gctgtcgagtaccgccg	6924-6940	ccaccaacagcactgagct	7624-7606	60
FECS I	accccgaggtcctttgagc	5485-5503	gctgtgcgcaaaagccct	5846-5828	62
FECS II	gaaccattgcttgaggg	4374-4390	ggtaacctcttagtcatctgtattc	4752-4728	58
FECS III	gcagccgccgttccagc	3880-3896	ggaacccgactatctgcc	4155-4138	56
FECS IV	tgcaagttagcttcccaa	2960-2978	caccaagtgtgggaaagag	3351-3333	58
FECS V	catgctctctggttgtcattattca	2436-2460	cgcagtgactcagaaacactt	2760-2736	60
FECS VI	ggcctggaaggccctatac	1056-1074	tacaggtgtgagccaccgc	1272-1254	58
FECS VII	tgcgatttgtgggttctcg	633-651	atacccggttccaggcct	1072-1055	62
FECS VIII	ccagtcacacgcttgcc	126-142	cgagaacccacaaatcgc	651-634	60
FECS IX	cgagacttggagtgcg	7785-7801	cgccaaacgagcgga	8181-8167	58

	Forward primer (5′→3′)	Location	Reverse primer (5′→3′)	Location	Annealing temperature, °C
Coding region-1	cgacggccaatcgga	5639-5653	ggctcggtttcaggagtttg	6112-6193	60
Coding region-2	tggctgctacccgcc	5854-5868	gagacgcgccggtgt	6235-6221	62
Coding region-3	gctcggacaccggcg	6215-6229	gttggtgtaaacggg	6549-6528	62
Coding region-4	cccgtttacaccaactca	6535-6553	cttcaccttgtcctccagg	6900-6882	64
Coding region-5	gctgtcgagtaccgccg	6924-6940	ccaccaacagcactgagct	7624-7606	60
FECS I	accccgaggtcctttgagc	5485-5503	gctgtgcgcaaaagccct	5846-5828	62
FECS II	gaaccattgcttgaggg	4374-4390	ggtaacctcttagtcatctgtattc	4752-4728	58
FECS III	gcagccgccgttccagc	3880-3896	ggaacccgactatctgcc	4155-4138	56
FECS IV	tgcaagttagcttcccaa	2960-2978	caccaagtgtgggaaagag	3351-3333	58
FECS V	catgctctctggttgtcattattca	2436-2460	cgcagtgactcagaaacactt	2760-2736	60
FECS VI	ggcctggaaggccctatac	1056-1074	tacaggtgtgagccaccgc	1272-1254	58
FECS VII	tgcgatttgtgggttctcg	633-651	atacccggttccaggcct	1072-1055	62
FECS VIII	ccagtcacacgcttgcc	126-142	cgagaacccacaaatcgc	651-634	60
FECS IX	cgagacttggagtgcg	7785-7801	cgccaaacgagcgga	8181-8167	58

The locations indicated are base numbers according to the human JunB gene sequence (GenBank accession no.U20734).