Description of AT-rich regions present in each 5′HS3 β/γ-globin hybrid transgene and a summary of transgenic animals and transgene expression levels
| Transgene . | AT-rich region . | Mice expressing single-copy . | Mice expressing total . | Mean expression* ± standard error (SE) . | Difference from BGT50‡ . |
|---|---|---|---|---|---|
| BGT50 | Wild-type | 7/7 | 8/8 | 64 ± 11 | NA |
| BGT64 | ΔAT-rich region | 2/4 | 13/17 | 31† ± 2 | P < .01 |
| BGT131 | mOct-1 | 3/3 | 10/10 | 31 ± 8 | P< .05 |
| BGT132 | mGata-1 | — | 9/9 | 61 ± 12 | P> .50 |
| BGT133 | mGata-1/mOct-1 | 7/7 | 18/18 | 37 ± 8 | P< .05 |
| Transgene . | AT-rich region . | Mice expressing single-copy . | Mice expressing total . | Mean expression* ± standard error (SE) . | Difference from BGT50‡ . |
|---|---|---|---|---|---|
| BGT50 | Wild-type | 7/7 | 8/8 | 64 ± 11 | NA |
| BGT64 | ΔAT-rich region | 2/4 | 13/17 | 31† ± 2 | P < .01 |
| BGT131 | mOct-1 | 3/3 | 10/10 | 31 ± 8 | P< .05 |
| BGT132 | mGata-1 | — | 9/9 | 61 ± 12 | P> .50 |
| BGT133 | mGata-1/mOct-1 | 7/7 | 18/18 | 37 ± 8 | P< .05 |
NA indicates not applicable; —, no single copy transgenic mice obtained.
Mean expression listed as the mean of the percentage of human γ-globin transcripts to endogenous mouse βmajor-globin transcripts on a per copy basis of each mouse.
Calculated from expression levels of the 13 expressing mice.
Calculated by the Wilcoxon rank sum method.