Flow cytometry was used to measure the expression level of FTVII by binding of the anti-sLe^x mAb CSLEX-1. The expression level of Core2GlcNAcT-I was measured by binding of a mAb to the T antigen to enzymatically desialylated cells. Core-2O-glycans mask the epitopes for the mAb to the T antigen, resulting in decreased mAb binding to cell surfaces. The mAb binding was detected by binding of FITC-conjugated goat antimouse IgG/IgM. The data are represented as the MFI. MFI indicates mean fluorescence intensity; ND, not determined.