Hemodynamic parameters of the mesenteric venules investigated
| Mouse type . | Animal treatment . | No. of Venules . | Vessel diameter, μm . | RBC velocity, μm/s . | Calculated WSR, s−1 . |
|---|---|---|---|---|---|
| WT | Sham | 6 | 28.0 ± 1.4 | 2.58 ± 0.55 | 454 ± 79 |
| WT | PBS | 18 | 24.5 ± 1.7 | 1.32 ± 0.28 | 271 ± 20 |
| WT | Ac2-26 (20 μg) | 12 | 24.8 ± 1.3 | 1.39 ± 0.05 | 287 ± 15 |
| WT | Ac2-26 (100 μg) | 10 | 25.8 ± 1.3 | 1.86 ± 0.13 | 363 ± 17 |
| WT | Boc2 | 6 | 24.6 ± 1.5 | 1.58 ± 0.11 | 327 ± 39 |
| WT | Ac2-26 + Boc2 | 7 | 30.4 ± 2.9 | 1.56 ± 0.07 | 286 ± 26 |
| FPR KO | Sham | 5 | 26.6 ± 2.9 | 1.8 ± 0.32 | 368 ± 78 |
| FPR KO | PBS | 7 | 23.5 ± 0.7 | 1.8 ± 0.19 | 379 ± 50 |
| FPR KO | Ac2-26 (100 μg) | 9 | 28.0 ± 3.4 | 2.0 ± 0.27 | 354 ± 12 |
| FPR KO | Ac2-26 + Boc2 | 10 | 26.6 ± 1.5 | 1.9 ± 0.11 | 361 ± 20 |
| Mouse type . | Animal treatment . | No. of Venules . | Vessel diameter, μm . | RBC velocity, μm/s . | Calculated WSR, s−1 . |
|---|---|---|---|---|---|
| WT | Sham | 6 | 28.0 ± 1.4 | 2.58 ± 0.55 | 454 ± 79 |
| WT | PBS | 18 | 24.5 ± 1.7 | 1.32 ± 0.28 | 271 ± 20 |
| WT | Ac2-26 (20 μg) | 12 | 24.8 ± 1.3 | 1.39 ± 0.05 | 287 ± 15 |
| WT | Ac2-26 (100 μg) | 10 | 25.8 ± 1.3 | 1.86 ± 0.13 | 363 ± 17 |
| WT | Boc2 | 6 | 24.6 ± 1.5 | 1.58 ± 0.11 | 327 ± 39 |
| WT | Ac2-26 + Boc2 | 7 | 30.4 ± 2.9 | 1.56 ± 0.07 | 286 ± 26 |
| FPR KO | Sham | 5 | 26.6 ± 2.9 | 1.8 ± 0.32 | 368 ± 78 |
| FPR KO | PBS | 7 | 23.5 ± 0.7 | 1.8 ± 0.19 | 379 ± 50 |
| FPR KO | Ac2-26 (100 μg) | 9 | 28.0 ± 3.4 | 2.0 ± 0.27 | 354 ± 12 |
| FPR KO | Ac2-26 + Boc2 | 10 | 26.6 ± 1.5 | 1.9 ± 0.11 | 361 ± 20 |
WT or FPR KO mice received either PBS (0.1 mL), peptide Ac2-26 (20 or 100 μg, corresponding to 6.6 nmol or 33 nmol), Boc 2 (10 μg or 12 nmol), or a combination of Ac2-26 + Boc 2 (100 μg + 10 μg, respectively) injected intravenously at the start of reperfusion (R = 0 minutes). A sham group was also included. Centerline (or red blood cell [RBC]) velocity was quantified at 45 minutes after reperfusion. Calculated wall shear rate (WSR) was determined from RBC velocity and vessel diameter as described in “Materials and methods.” Data are mean ± SEM of n = 4 to 6 mice per group.