Okadaic acid enhances monocytic differentiation of HL-60 cells
| Treatment . | CD14+CD15−, % . | CD15+CD14−, % . | NSE+, %3-150 . |
|---|---|---|---|
| 2d C, 2d C | 1, 1 | 84, 87 | 0, 0 |
| 2d C, 2d OA | 39, 32 | 7, 11 | 35, 32 |
| 2d ATRA, 2d C | 1, 2 | 79, 84 | 0, 3 |
| 2d ATRA, 2d OA | 29, 27 | 8, 11 | 62, 47 |
| 2d ATRA, 2d ATRA | 1, 3 | 84, 91 | 0, 0 |
| 2d ATRA, 2d ATRA + OA | 23, 24 | 11, 19 | 47, 38 |
| 2d TGF-β, 2dC | 6, 8 | 75, 71 | 37, 39 |
| 2d TGF-β, 2d OA | 43, 37 | 9, 14 | 74, 69 |
| 2d TGF-β + ATRA, 2d TGF-β + ATRA | 31, 35 | 63, 55 | 65, 57 |
| 2d TGF-β + ATRA, 2d TGF-β + ATRA + OA | 51, 52 | 22, 28 | 81, 91 |
| Treatment . | CD14+CD15−, % . | CD15+CD14−, % . | NSE+, %3-150 . |
|---|---|---|---|
| 2d C, 2d C | 1, 1 | 84, 87 | 0, 0 |
| 2d C, 2d OA | 39, 32 | 7, 11 | 35, 32 |
| 2d ATRA, 2d C | 1, 2 | 79, 84 | 0, 3 |
| 2d ATRA, 2d OA | 29, 27 | 8, 11 | 62, 47 |
| 2d ATRA, 2d ATRA | 1, 3 | 84, 91 | 0, 0 |
| 2d ATRA, 2d ATRA + OA | 23, 24 | 11, 19 | 47, 38 |
| 2d TGF-β, 2dC | 6, 8 | 75, 71 | 37, 39 |
| 2d TGF-β, 2d OA | 43, 37 | 9, 14 | 74, 69 |
| 2d TGF-β + ATRA, 2d TGF-β + ATRA | 31, 35 | 63, 55 | 65, 57 |
| 2d TGF-β + ATRA, 2d TGF-β + ATRA + OA | 51, 52 | 22, 28 | 81, 91 |
Okadaic acid concentration is 2.5 nM; TGF-β is 10 ng/mL; ATRA is 100 nM for the first 2 days of incubation and 10 nM for the second 2 days of incubation. Experiments are done in 5% serum. Individual values are shown for 2 separate experiments. 2d indicates second 2 days of incubation; C, control medium; and OA, okadaic acid.
500 cells were counted.