Effect of U0126 on the surface expression of CD11c
| Experiment no. . | Percentage of cells CD11c+ . | Percentage drop in cells CD11c+ . | |
|---|---|---|---|
| Untreated . | U0126 . | ||
| 1 | 17 | 2.5 | 85.3 |
| 2 | 2.3 | 1.5 | 34.8 |
| 3 | 5.6 | 4.3 | 23.2 |
| 4 | 8.5 | 1.3 | 84.7 |
| 5 | 4.5 | 3.0 | 33.3 |
| 6 | 4.5 | 2.5 | 44.4 |
| 7 | 4.1 | 3.1 | 24.4 |
| 8 | 4.2 | 3.2 | 23.8 |
| Experiment no. . | Percentage of cells CD11c+ . | Percentage drop in cells CD11c+ . | |
|---|---|---|---|
| Untreated . | U0126 . | ||
| 1 | 17 | 2.5 | 85.3 |
| 2 | 2.3 | 1.5 | 34.8 |
| 3 | 5.6 | 4.3 | 23.2 |
| 4 | 8.5 | 1.3 | 84.7 |
| 5 | 4.5 | 3.0 | 33.3 |
| 6 | 4.5 | 2.5 | 44.4 |
| 7 | 4.1 | 3.1 | 24.4 |
| 8 | 4.2 | 3.2 | 23.8 |
The hairy cell line EH was cultured for 48 hours in the absence of treatment or, in parallel, with 8 μM U0126. Flow cytometric analysis was then performed using a CD11c-specific antibody and an isotype-matched control. The number of cells bound by the control antibody was subtracted from the number bound by the CD11c antibody, such that the percentage of cells specifically bound by the CD11c antibody could be calculated. This percentage, derived from cells untreated or treated with U0126, is presented from a total of 8 independent experiments. Also presented is the percentage drop caused by U0126 in the number of cells expressing CD11c. The mean percentage drop in cells expressing CD11c was calculated as 44.2% with a standard deviation of ± 26.1%. The Student t test yielded a statistically significant probability value for the drop in CD11c expression of 0.03.