Effects of antibody incubation on lymphocyte adherence to skin specimens of acute cutaneous GVHD
| Antibody4-150 . | Mean (SEM) of binding (% of isotype control incubation)4-151 . |
|---|---|
| Anti–E-selectin | 99.7 (7.8) |
| Anti–P-selectin | 96.9 (6.7) |
| Anti–VCAM-1 | 103.0 (7.0) |
| Antihuman L-selectin (LAM1-3) | 95.8 (5.4) |
| Anti–LFA-1 | 96.1 (5.1) |
| Antibody4-150 . | Mean (SEM) of binding (% of isotype control incubation)4-151 . |
|---|---|
| Anti–E-selectin | 99.7 (7.8) |
| Anti–P-selectin | 96.9 (6.7) |
| Anti–VCAM-1 | 103.0 (7.0) |
| Antihuman L-selectin (LAM1-3) | 95.8 (5.4) |
| Anti–LFA-1 | 96.1 (5.1) |
Antibody concentration used in data reported here was 50 μg/mL.
Number of lymphocytes adherent to papillary dermis was quantified by using an ocular grid under × 400 magnification. Results are presented as percentage of binding compared with corresponding sequential sections incubated with isotope-matched control antibodies. Two grids per slide were examined, minimum of 2 slides per experiment, 4 separate experiments.