Effects of soluble CD100 (sCD100) on CLL cells viability and proliferation
| . | CLL cells alone . | + sCD100 . | + MLP . | + MLP + sCD100 . | + MLP/Plex-B1 . | + MLP/Plex-B1 + sCD100 . |
|---|---|---|---|---|---|---|
| Viability | ||||||
| Day 3 | 62.5 (± 9.2) | 61.5 (± 14.8) | 70.5 (± 10.6) | 66 (± 8.5) | 93.5 (± 3.5) | 71 (± 5.7) |
| Day 5 | 59 (± 14.1) | 57.5 (± 19.1) | 73.3 (± 2.1) | 62 (± 19.8) | 94 (± 1.4) | 64 (± 4.2) |
| Proliferation | ||||||
| Day 3 | 1.1 (± 1.0) | 1 (± 0.7) | 1.6 (± 0.6) | 1.7 (± 0.3) | 5.6 (± 1.6) | 1.6 (± 0.8) |
| Day 5 | 1 (± 0.6) | 1 (± 0.7) | 1.7 (± 0.4) | 1.8 (± 0.3) | 6 (± 1.6) | 2 (± 0.9) |
| . | CLL cells alone . | + sCD100 . | + MLP . | + MLP + sCD100 . | + MLP/Plex-B1 . | + MLP/Plex-B1 + sCD100 . |
|---|---|---|---|---|---|---|
| Viability | ||||||
| Day 3 | 62.5 (± 9.2) | 61.5 (± 14.8) | 70.5 (± 10.6) | 66 (± 8.5) | 93.5 (± 3.5) | 71 (± 5.7) |
| Day 5 | 59 (± 14.1) | 57.5 (± 19.1) | 73.3 (± 2.1) | 62 (± 19.8) | 94 (± 1.4) | 64 (± 4.2) |
| Proliferation | ||||||
| Day 3 | 1.1 (± 1.0) | 1 (± 0.7) | 1.6 (± 0.6) | 1.7 (± 0.3) | 5.6 (± 1.6) | 1.6 (± 0.8) |
| Day 5 | 1 (± 0.6) | 1 (± 0.7) | 1.7 (± 0.4) | 1.8 (± 0.3) | 6 (± 1.6) | 2 (± 0.9) |
sCD100 was added to the cultures and its effects on viability and proliferation were evaluated by Annexin V and PI staining, respectively. The numbers express the percentage of viable cells and of cells in S, G2, M phases of the cell cycle and represent the mean values ± standard deviations obtained in 2 independent experiments.