Differential counts of FDC-P1 cells transduced with control, hC/EBPα, or hC/EBPɛ retroviral constructs
| . | Immature/blasts . | Intermediate . | Segmented . |
|---|---|---|---|
| Control | 96.0 ± 1.2 | 4.0 ± 1.2 | 0.1 ± 0.2 |
| hC/EBPα | 63.8 ± 1.3 | 24.9 ± 0.8 | 11.3 ± 2.1 |
| hC/EBPɛ | 87.0 ± 4.4 | 12.8 ± 4.0 | 0.3 ± 0.6 |
| . | Immature/blasts . | Intermediate . | Segmented . |
|---|---|---|---|
| Control | 96.0 ± 1.2 | 4.0 ± 1.2 | 0.1 ± 0.2 |
| hC/EBPα | 63.8 ± 1.3 | 24.9 ± 0.8 | 11.3 ± 2.1 |
| hC/EBPɛ | 87.0 ± 4.4 | 12.8 ± 4.0 | 0.3 ± 0.6 |
At day 4 (6 days after the first of 2 rounds of transduction), GFP+ cells were sorted and cytospins were prepared and stained with Wright Giemsa. A total of 300 cells were counted for each cytospin. Results shown are means ± SDs of an experiment performed in triplicate. Differential counts of hC/EBPα samples are significantly different from control (immature/blasts, P = .001; intermediate, P = .001; segmented,P = .01). Differential counts of hC/EBPɛ samples trend toward significance compared with control (immature/blasts,P = .06; intermediate, P = .06; segmented,P = not significant).
Immature/blasts indicates immature cells with high nuclear to cytoplasmic ratios and round nuclei; intermediate, forms including ring forms, bands, and binucleated or trinucleated cells; segmented, cells with nuclear segmentation.