Table 1.

Implication of AID expression in mutational profile of Iμ/Sμ region, CSR, and SHM

Sample DNA and RNA stimulation, CD40L + IL4 for 5 daysAID transcripts, RT-PCRSHM homology to germline, %Iμ/Sμ regionClonal isotype switched transcripts, RT-PCR
5′ region3′ regionComplete region
MutationsDeletionsMutationsDeletionsMutations/bp sequencesVH/μVH/δVH/γVH/α
Healthy B cells            
 No − N/C 0 of 769 0 of 769 0 of 875 0 of 875 0 of 1625 − − − − 
 Yes + N/C 0 of 769 0 of 769 2 of 875 0 of 875 1.2 × 10−3 − − − −  
B-CLLs no. 1            
 No + 100 0 of 769 0 of 769 4 of 875 0 of 875 2.4 × 10−3 + + + + 
 Yes + 100 1 of 769 0 of 769 4 of 875 1 of 875 3.0 × 10−3 + + + +  
B-CLLs no. 2            
 No + 100 2 of 769 0 of 769 1 of 875 0 of 875 1.8 × 10−3 + + + − 
 Yes + 100 3 of 769 0 of 769 2 of 875 1 of 875 3.0 × 10−3 + − + −  
B-CLLs no. 3            
 No + 99 0 of 769 0 of 769 3 of 875 1 of 875 1.8 × 10−3 + + + − 
 Yes + 99 0 of 769 0 of 769 5 of 875 1 of 875 3.1 × 10−3 + + + −  
B-CLLs no. 4 
 No  + 99 2 of 769 0 of 769 2 of 875 0 of 875 2.4 × 10−3 + + + +  
B-CLLs no. 5            
 No − 95 0 of 769 0 of 769 0 of 875 0 of 875 0 of 1625 + + − −  
 Yes + 95 0 of 769 0 of 769 2 of 875 0 of 875 1.2 × 10−3 + + − +  
B-CLLs no. 6            
 No − 92 0 of 769 0 of 769 1 of 875 0 of 875 6.1 × 10−4 − − + − 
 Yes + 92 1 of 769 0 of 769 2 of 875 0 of 875 1.8 × 10−3 − − + −  
B-CLLs no. 7            
 No − 93 0 of 769 0 of 769 0 of 875 0 of 875 0 of 1625 + + − −  
 Yes + 93 0 of 769 0 of 769 2 of 875 0 of 875 1.2 × 10−3 + + + + 
Sample DNA and RNA stimulation, CD40L + IL4 for 5 daysAID transcripts, RT-PCRSHM homology to germline, %Iμ/Sμ regionClonal isotype switched transcripts, RT-PCR
5′ region3′ regionComplete region
MutationsDeletionsMutationsDeletionsMutations/bp sequencesVH/μVH/δVH/γVH/α
Healthy B cells            
 No − N/C 0 of 769 0 of 769 0 of 875 0 of 875 0 of 1625 − − − − 
 Yes + N/C 0 of 769 0 of 769 2 of 875 0 of 875 1.2 × 10−3 − − − −  
B-CLLs no. 1            
 No + 100 0 of 769 0 of 769 4 of 875 0 of 875 2.4 × 10−3 + + + + 
 Yes + 100 1 of 769 0 of 769 4 of 875 1 of 875 3.0 × 10−3 + + + +  
B-CLLs no. 2            
 No + 100 2 of 769 0 of 769 1 of 875 0 of 875 1.8 × 10−3 + + + − 
 Yes + 100 3 of 769 0 of 769 2 of 875 1 of 875 3.0 × 10−3 + − + −  
B-CLLs no. 3            
 No + 99 0 of 769 0 of 769 3 of 875 1 of 875 1.8 × 10−3 + + + − 
 Yes + 99 0 of 769 0 of 769 5 of 875 1 of 875 3.1 × 10−3 + + + −  
B-CLLs no. 4 
 No  + 99 2 of 769 0 of 769 2 of 875 0 of 875 2.4 × 10−3 + + + +  
B-CLLs no. 5            
 No − 95 0 of 769 0 of 769 0 of 875 0 of 875 0 of 1625 + + − −  
 Yes + 95 0 of 769 0 of 769 2 of 875 0 of 875 1.2 × 10−3 + + − +  
B-CLLs no. 6            
 No − 92 0 of 769 0 of 769 1 of 875 0 of 875 6.1 × 10−4 − − + − 
 Yes + 92 1 of 769 0 of 769 2 of 875 0 of 875 1.8 × 10−3 − − + −  
B-CLLs no. 7            
 No − 93 0 of 769 0 of 769 0 of 875 0 of 875 0 of 1625 + + − −  
 Yes + 93 0 of 769 0 of 769 2 of 875 0 of 875 1.2 × 10−3 + + + + 

Results of 3 independent colony experiments are shown together.

N/C indicates no clonal VDJ gene

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