Ectopic HOXB4 expression impairs lymphoid and myeloid differentiation in vivo
| Group and compartment . | No. mice3-150 . | Proportion (%) of human leukocytes . | |||
|---|---|---|---|---|---|
| CD19+ and CD33+combined . | CD19+, lymphoid . | CD33+, myeloid . | CD34+ . | ||
| HOXB4 | |||||
| GFP+ | 8 | 69.5 ± 6.33-151 | 48.7 ± 3.63-151 | 19.4 ± 4.73-152 | 26.5 ± 5.33-151 |
| GFP− | 8 | 82.3 ± 3.8 | 57.6 ± 3.9 | 23.8 ± 2.4 | 14.6 ± 2.6 |
| RFP | |||||
| RFP+ | 8 | 67.5 ± 9.0 | 36.1 ± 5.6 | 31.4 ± 6.5 | 16.6 ± 5.3 |
| RFP− | 8 | 54.8 ± 3.5 | 30.9 ± 4.6 | 23.8 ± 3.0 | 10.4 ± 2.5 |
| Group and compartment . | No. mice3-150 . | Proportion (%) of human leukocytes . | |||
|---|---|---|---|---|---|
| CD19+ and CD33+combined . | CD19+, lymphoid . | CD33+, myeloid . | CD34+ . | ||
| HOXB4 | |||||
| GFP+ | 8 | 69.5 ± 6.33-151 | 48.7 ± 3.63-151 | 19.4 ± 4.73-152 | 26.5 ± 5.33-151 |
| GFP− | 8 | 82.3 ± 3.8 | 57.6 ± 3.9 | 23.8 ± 2.4 | 14.6 ± 2.6 |
| RFP | |||||
| RFP+ | 8 | 67.5 ± 9.0 | 36.1 ± 5.6 | 31.4 ± 6.5 | 16.6 ± 5.3 |
| RFP− | 8 | 54.8 ± 3.5 | 30.9 ± 4.6 | 23.8 ± 3.0 | 10.4 ± 2.5 |
Chimeric bone marrow samples were stained with various monoclonal antibodies specific against different human blood cell lineages and CD45 as a marker for human cells. The gated human CD45+ cells were analyzed for GFP expression and subsequently for expression of human CD19 (B lymphoid), CD33 (myeloid), or CD34. Percentages of GFP+ and GFP−lymphoid, myeloid, and CD34+ cells, respectively, are presented as percentages of the human GFP+ and GFP− CD45+ cell population.
Mice from both independent transplantation experiments were combined for the analysis. Statistical analysis was performed by applying the paired, 2-sided Student t test.
P < .01.
In 4 of 8 mice receiving transplants of HOXB4, the proportion of CD33+ cells in the GFP+ population was significantly lower than in the GFP− population: 8.3 ± 1.9 versus 22.3 ± 4.5, respectively; n = 4;P = .01.