Ectopic HOXB4 expression confers a selective growth advantage on human NOD/SCID repopulating cells in vivo
| Experiment and group . | No. mice . | G(R)FP+ as % of CD34+in input cells . | Analysis of engraftment . | |||
|---|---|---|---|---|---|---|
| G(R)FP+ as % of CD34+ . | CD34+ . | % CD45* . | ||||
| As % of G(R)FP+ . | As % of G(R)FP− . | |||||
| No. 1 | ||||||
| HOX | 4 | 33.3 | 36.1 ± 12.1 | 18.0 ± 2.0† | 9.5 ± 1.7 | 4.5 ± 0.6 |
| RFP | 5 | 76.0 | 44.2 ± 13.1 | 6.2 ± 1.1 | 6.0 ± 2.0 | 3.2 ± 1.7 |
| No. 2 | ||||||
| HOX | 4 | 67.9 | 80.2 ± 9.2† | 35.0 ± 8.9† | 19.6 ± 3.5 | 6.0 ± 1.8 |
| RFP | 3 | 63.3 | 3.12 ± 1.0 | 23.7 ± 5.0 | 17.8 ± 0.7 | 1.3 ± 0.8 |
| Experiment and group . | No. mice . | G(R)FP+ as % of CD34+in input cells . | Analysis of engraftment . | |||
|---|---|---|---|---|---|---|
| G(R)FP+ as % of CD34+ . | CD34+ . | % CD45* . | ||||
| As % of G(R)FP+ . | As % of G(R)FP− . | |||||
| No. 1 | ||||||
| HOX | 4 | 33.3 | 36.1 ± 12.1 | 18.0 ± 2.0† | 9.5 ± 1.7 | 4.5 ± 0.6 |
| RFP | 5 | 76.0 | 44.2 ± 13.1 | 6.2 ± 1.1 | 6.0 ± 2.0 | 3.2 ± 1.7 |
| No. 2 | ||||||
| HOX | 4 | 67.9 | 80.2 ± 9.2† | 35.0 ± 8.9† | 19.6 ± 3.5 | 6.0 ± 1.8 |
| RFP | 3 | 63.3 | 3.12 ± 1.0 | 23.7 ± 5.0 | 17.8 ± 0.7 | 1.3 ± 0.8 |
CD34+ cells were transduced and injected into NOD/SCID mice 24 hours after the last infection. Mice were killed 8 weeks later, and bone marrow was analyzed for human cells expressing GFP and/or RFP. Results are shown as the mean ± SE.
Proportion of all viable (PI−) cells in retrieved bone marrow of the chimeric mice.
Student t test (paired; 2-sided). P < .05.