Frequency of clonogenic progenitors in populations of highly enriched CD133+ cells, highly enriched CD34+ cells, and nonadherent CD34− cells
| Cell fraction . | No. CFCs per 1000 cells, mean ± SEM . | Frequency of CAFCs . | |
|---|---|---|---|
| Week 2 . | Week 6 . | ||
| CD133+ | 187 ± 35; n = 6 | 1 among 205; n = 5 | 1 among 154; n = 5 |
| CD34+ | 225 ± 65; n = 8 | 1 among 321; n = 5 | 1 among 178; n = 5 |
| NA CD34− | ND; n = 4 | ND; n = 4 | ND; n = 4 |
| Cell fraction . | No. CFCs per 1000 cells, mean ± SEM . | Frequency of CAFCs . | |
|---|---|---|---|
| Week 2 . | Week 6 . | ||
| CD133+ | 187 ± 35; n = 6 | 1 among 205; n = 5 | 1 among 154; n = 5 |
| CD34+ | 225 ± 65; n = 8 | 1 among 321; n = 5 | 1 among 178; n = 5 |
| NA CD34− | ND; n = 4 | ND; n = 4 | ND; n = 4 |
Human CFCs were assayed in methylcellulose by standard methods. Progenitors were quantified by CAFC assays with serial cell dilutions (32-1000 cells per well). The percentage of wells containing at least one phase-dark hematopoietic clone beneath the stromal layer was determined each week for 6 weeks, and the frequency of CAFCs was calculated by likelihood maximization.
SEM indicates standard error of the mean; ND, not detected.