Phenotype analysis of DCs matured after exposure to LPS in the presence of IVIg
| Treatment . | CD83 . | HLA-DR . | CD86 . | CD80 . | CD40 . | CD95 . |
|---|---|---|---|---|---|---|
| Ctl-LPS | 30 ± 2 | 208 ± 32 | 84 ± 16 | 53 ± 7 | 381 ± 34 | 46 ± 9 |
| IVIg-LPS | 21 ± 43-150 | 156 ± 50 | 54 ± 103-150 | 40 ± 43-150 | 210 ± 326 | 34 ± 10 |
| HSA-LPS | 29 ± 4 | 173 ± 12 | 111 ± 4 | 56 ± 5 | 404 ± 156 | 46 ± 2 |
| Treatment . | CD83 . | HLA-DR . | CD86 . | CD80 . | CD40 . | CD95 . |
|---|---|---|---|---|---|---|
| Ctl-LPS | 30 ± 2 | 208 ± 32 | 84 ± 16 | 53 ± 7 | 381 ± 34 | 46 ± 9 |
| IVIg-LPS | 21 ± 43-150 | 156 ± 50 | 54 ± 103-150 | 40 ± 43-150 | 210 ± 326 | 34 ± 10 |
| HSA-LPS | 29 ± 4 | 173 ± 12 | 111 ± 4 | 56 ± 5 | 404 ± 156 | 46 ± 2 |
Immature DCs obtained after 5-day culture with IL-4 and GM-CSF were treated with IVIg (0.15 mM) for 12 hours followed by stimulation with LPS (1 μg/mL) (IVIg-LPS) or were treated with LPS alone (Ctl-LPS) for 48 hours. In control experiments, cells were treated with HSA, an irrelevant protein, for 12 hours followed by stimulation with LPS (HSA-LPS). Results are expressed as MFI. Data are from 4 independent experiments from different donors. Statistical significance as determined by unpaired Student t test is indicated.
P < .05.