ΔMFI of surface molecules on Mo-DCs and ActMo-DCs
| Dendritic cell (DC) type . | HLA-DR . | CD86 . | CD40 . | CD83 . |
|---|---|---|---|---|
| Mo-DCs* | 1396 ± 210 | 1857 ± 1435 | 2259 ± 1224 | 60 ± 17 |
| ActMo-DCs (LPS)* | 2694 ± 274 | 3549 ± 2965 | 4732 ± 1775 | 69 ± 6 |
| ActMo-DCs (MCM)* | 1752 ± 536 | 2091 ± 1425 | 3006 ± 1173 | 72 ± 7 |
| Mo-DCs† | 1010 ± 98 | 2950 ± 845 | 2238 ± 1018 | 125 ± 47 |
| ActMo-DCs (LPS)† | 2940 ± 181 | 5353 ± 1771 | 3781 ± 1616 | 95 ± 19 |
| ActMo-DCs (MCM)† | 2025 ± 498 | 3991 ± 417 | 3401 ± 1943 | 102 ± 17 |
| Dendritic cell (DC) type . | HLA-DR . | CD86 . | CD40 . | CD83 . |
|---|---|---|---|---|
| Mo-DCs* | 1396 ± 210 | 1857 ± 1435 | 2259 ± 1224 | 60 ± 17 |
| ActMo-DCs (LPS)* | 2694 ± 274 | 3549 ± 2965 | 4732 ± 1775 | 69 ± 6 |
| ActMo-DCs (MCM)* | 1752 ± 536 | 2091 ± 1425 | 3006 ± 1173 | 72 ± 7 |
| Mo-DCs† | 1010 ± 98 | 2950 ± 845 | 2238 ± 1018 | 125 ± 47 |
| ActMo-DCs (LPS)† | 2940 ± 181 | 5353 ± 1771 | 3781 ± 1616 | 95 ± 19 |
| ActMo-DCs (MCM)† | 2025 ± 498 | 3991 ± 417 | 3401 ± 1943 | 102 ± 17 |
Δ Mean fluorescence intensity (ΔMFI) was calculated by subtracting the MFI of isotype-matched control from that of the indicated surface molecules. The data are representative of 3 experiments and presented as mean ± SD.
Mo-DCs indicates monocyte-derived dendritic cells; ActMo-DCs, activated/differentiated Mo-DCs; LPS, lipopolysaccharide; MCM, monocyte conditioned medium.
DCs generated in RPMI/5% AS, 200 U/mL GM-CSF, and 50 U/mL IL-4.
DCs generated in RPMI/1% AS, 1000 U/mL GM-CSF, and 1000 U/mL IL-4.