Effects of combined exposure to UCN-01 and PD184352 on apoptosis and loss of Δψm in MM cells
| . | Apoptotic cells (%) . | “Low” Δψm (% cells) . |
|---|---|---|
| 8226 | ||
| Control | 5.9 ± 2.7 | 2.8 ± 1.0 |
| PD184352 | 12.7 ± 2.6 | 12.6 ± 1.6 |
| UCN-01 | 9.1 ± 5.1 | 8.4 ± 1.3 |
| PD + UCN | 61.9 ± 5.9 | 39.6 ± 2.7 |
| H929 | ||
| Control | 3.6 ± 2.6 | 3.5 ± 1.4 |
| PD184352 | 10.0 ± 4.5 | 8.7 ± 0.1 |
| UCN-01 | 5.9 ± 1.9 | 6.8 ± 0.2 |
| PD + UCN | 73.8 ± 2.9 | 44.8 ± 0.9 |
| U266 | ||
| Control | 3.5 ± 0.8 | 3.2 ± 0.4 |
| PD184352 | 10.5 ± 1.7 | 8.1 ± 0.3 |
| UCN-01 | 7.5 ± 2.2 | 8.6 ± 0.1 |
| PD + UCN | 56.5 ± 11.6 | 37.8 ± 8.0 |
| . | Apoptotic cells (%) . | “Low” Δψm (% cells) . |
|---|---|---|
| 8226 | ||
| Control | 5.9 ± 2.7 | 2.8 ± 1.0 |
| PD184352 | 12.7 ± 2.6 | 12.6 ± 1.6 |
| UCN-01 | 9.1 ± 5.1 | 8.4 ± 1.3 |
| PD + UCN | 61.9 ± 5.9 | 39.6 ± 2.7 |
| H929 | ||
| Control | 3.6 ± 2.6 | 3.5 ± 1.4 |
| PD184352 | 10.0 ± 4.5 | 8.7 ± 0.1 |
| UCN-01 | 5.9 ± 1.9 | 6.8 ± 0.2 |
| PD + UCN | 73.8 ± 2.9 | 44.8 ± 0.9 |
| U266 | ||
| Control | 3.5 ± 0.8 | 3.2 ± 0.4 |
| PD184352 | 10.5 ± 1.7 | 8.1 ± 0.3 |
| UCN-01 | 7.5 ± 2.2 | 8.6 ± 0.1 |
| PD + UCN | 56.5 ± 11.6 | 37.8 ± 8.0 |
8226, H929, and U266 MM cells were exposed for 24 hours to 10 μM PD184352 + 150 nM UCN-01, after which the percentage of morphologically apoptotic cells was determined by evaluating Wright-Giemsa–stained cytospin preparations (left column) as described in “Materials and methods”. Alternatively, cells were treated as above, after which loss of mitochondrial membrane potential (Δψm) was monitored by analyzing DiOC6-treated cells by flow cytometry (right column) as described in “Materials and methods”. Values correspond to the percentage of cells displaying “low” DiOC6 uptake. In each case, values represent the mean ± SD for 3 separate experiments performed in triplicate.