Primers used in the 5′RACE experiments
| Experiment set . | Primer name . | Sequence . | Purpose . |
|---|---|---|---|
| First set | JP170 | 5′-GCCAGCAAATTCCAAGAG-3′ | Reverse transcription |
| JP164 | 5′-ATCGCAGGGTCACCGTTTCACCCGGC-3′ | First PCR | |
| JP134 | 5′-GACCATGCCAAACTGTGGCCAGCA-3′ | Second PCR | |
| Second set | JP170 | 5′-GCCAGCAAATTCCAAGAG-3′ | Reverse transcription |
| JP169 | 5′-AAGCTCGGTACAACGGTA-3′ | First PCR | |
| JP164 | 5′-ATCGCAGGGTCACCGTTTCACCCGGC-3′ | Second PCR | |
| Third set | JP193 | 5′-CCAGGGGATAGCGTGAGC-3′ | Reverse transcription |
| JP192 | 5-GATGCAGGTTGGAGAGTCG-3′ | PCR | |
| Fourth set | JP194 | 5′-AGGCCCCAACTCCATAGC-3′ | Reverse transcription |
| JP193 | 5′-CCAGGGGATAGCGTGAGC-3′ | PCR |
| Experiment set . | Primer name . | Sequence . | Purpose . |
|---|---|---|---|
| First set | JP170 | 5′-GCCAGCAAATTCCAAGAG-3′ | Reverse transcription |
| JP164 | 5′-ATCGCAGGGTCACCGTTTCACCCGGC-3′ | First PCR | |
| JP134 | 5′-GACCATGCCAAACTGTGGCCAGCA-3′ | Second PCR | |
| Second set | JP170 | 5′-GCCAGCAAATTCCAAGAG-3′ | Reverse transcription |
| JP169 | 5′-AAGCTCGGTACAACGGTA-3′ | First PCR | |
| JP164 | 5′-ATCGCAGGGTCACCGTTTCACCCGGC-3′ | Second PCR | |
| Third set | JP193 | 5′-CCAGGGGATAGCGTGAGC-3′ | Reverse transcription |
| JP192 | 5-GATGCAGGTTGGAGAGTCG-3′ | PCR | |
| Fourth set | JP194 | 5′-AGGCCCCAACTCCATAGC-3′ | Reverse transcription |
| JP193 | 5′-CCAGGGGATAGCGTGAGC-3′ | PCR |