Correlation between c-Rel expression and RELgenomic status as detected by immunohistology, CGH, and FICTION
| Case no.* . | Subtype of cHL . | Immunohistology of HRS cells . | Partial CGH karyotype (2p) . | FICTION data . | |||
|---|---|---|---|---|---|---|---|
| Nucleus . | Cytoplasm . | c-Rel expression . | REL/D2Z1ratio, median (range)† . | REL genomic status . | |||
| 1 | LR | − | − | Balanced | Slightly positive | 1.0 (1.0-1.0) | Intact |
| 2 | LR | − | − | Balanced | Negative | 1.0 (1.0-1.0) | Intact |
| 3 | LR | + | +++ | Balanced | NE | 1.0 (1.0-1.2)‡ | Intact |
| 4 | LR | ++ | − | Balanced | NE | NE | — |
| 5 | LR | ++ | + | rev ish enh(2p13-p21) | Positive | 2.0 (2.0-2.0) | Duplicated§ |
| 6 | LR | ++ | +++ | Balanced | NE | NE | — |
| 7 | MC | +++ | +++ | rev ish enh(2p13-p25) | ND | ND | — |
| 8 | MC | + | +++ | Balanced | NE | NE | — |
| 9 | MC | + | − | Balanced | Negative | 1.0 (1.0-1.5) | Intact |
| 10 | MC | + | ++ | Balanced | NE | NE | — |
| 11 | MC | NE | NE | Balanced | Negative | 1.0 (1.0-1.0) | Intact |
| 12 | MC | ++ | + | rev ish enh(2p11-p25) | NE | NE | — |
| 13 | MC | NE | NE | Balanced | Negative | 1.0 (1.0-2.0) | Intact |
| 14 | MC | ++ | +++ | Balanced | NE | 5.0 (1.0-6.5)‡ | Amplified |
| 15 | MC | +++ | − | rev ish amp(2p16) | Positive | 6.0 (2.0-6.5) | Amplified |
| 16a | NS | +++ | ++ | rev ish enh(2p12-p24) | ND | ND | — |
| 16b | NS | ++ | ++ | rev ish enh(2p13-p24) | Positive | 1.3 (1.0-1.7) | Duplicated |
| 17 | NS | ++ | ++ | rev ish enh(2p11-p24) | NE | NE | — |
| 18 | NS | +++ | +++ | rev ish enh(2p11-p25) | Positive | 1.5 (1.2-2.0) | Duplicated |
| 19 | NS | +++ | − | rev ish enh(2p12-p21) | Positive | 1.4 (1.2-1.8) | Duplicated |
| 20 | NS | ++ | +++ | Balanced | Positive | 1.0 (1.0-1.0) | Intact |
| 21 | NS | NE | NE | Balanced | Negative | 1.0 (1.0-1.0) | Intact |
| 22 | NS | +++ | +++ | rev ish enh(2p11-p25) | ND | ND | — |
| 23 | NS | ++ | + | rev ish enh(2p11-p25) | Positive | 1.5 (1.4-1.7) | Duplicated§ |
| 24 | NS | +++ | +++ | rev ish amp (2p13-p16) | Positive | 7.5 (5.0-10.0) | Amplified |
| 25 | LD | + | − | Balanced | Slightly positive | 1.0 (1.0-1.0) | Intact |
| Case no.* . | Subtype of cHL . | Immunohistology of HRS cells . | Partial CGH karyotype (2p) . | FICTION data . | |||
|---|---|---|---|---|---|---|---|
| Nucleus . | Cytoplasm . | c-Rel expression . | REL/D2Z1ratio, median (range)† . | REL genomic status . | |||
| 1 | LR | − | − | Balanced | Slightly positive | 1.0 (1.0-1.0) | Intact |
| 2 | LR | − | − | Balanced | Negative | 1.0 (1.0-1.0) | Intact |
| 3 | LR | + | +++ | Balanced | NE | 1.0 (1.0-1.2)‡ | Intact |
| 4 | LR | ++ | − | Balanced | NE | NE | — |
| 5 | LR | ++ | + | rev ish enh(2p13-p21) | Positive | 2.0 (2.0-2.0) | Duplicated§ |
| 6 | LR | ++ | +++ | Balanced | NE | NE | — |
| 7 | MC | +++ | +++ | rev ish enh(2p13-p25) | ND | ND | — |
| 8 | MC | + | +++ | Balanced | NE | NE | — |
| 9 | MC | + | − | Balanced | Negative | 1.0 (1.0-1.5) | Intact |
| 10 | MC | + | ++ | Balanced | NE | NE | — |
| 11 | MC | NE | NE | Balanced | Negative | 1.0 (1.0-1.0) | Intact |
| 12 | MC | ++ | + | rev ish enh(2p11-p25) | NE | NE | — |
| 13 | MC | NE | NE | Balanced | Negative | 1.0 (1.0-2.0) | Intact |
| 14 | MC | ++ | +++ | Balanced | NE | 5.0 (1.0-6.5)‡ | Amplified |
| 15 | MC | +++ | − | rev ish amp(2p16) | Positive | 6.0 (2.0-6.5) | Amplified |
| 16a | NS | +++ | ++ | rev ish enh(2p12-p24) | ND | ND | — |
| 16b | NS | ++ | ++ | rev ish enh(2p13-p24) | Positive | 1.3 (1.0-1.7) | Duplicated |
| 17 | NS | ++ | ++ | rev ish enh(2p11-p24) | NE | NE | — |
| 18 | NS | +++ | +++ | rev ish enh(2p11-p25) | Positive | 1.5 (1.2-2.0) | Duplicated |
| 19 | NS | +++ | − | rev ish enh(2p12-p21) | Positive | 1.4 (1.2-1.8) | Duplicated |
| 20 | NS | ++ | +++ | Balanced | Positive | 1.0 (1.0-1.0) | Intact |
| 21 | NS | NE | NE | Balanced | Negative | 1.0 (1.0-1.0) | Intact |
| 22 | NS | +++ | +++ | rev ish enh(2p11-p25) | ND | ND | — |
| 23 | NS | ++ | + | rev ish enh(2p11-p25) | Positive | 1.5 (1.4-1.7) | Duplicated§ |
| 24 | NS | +++ | +++ | rev ish amp (2p13-p16) | Positive | 7.5 (5.0-10.0) | Amplified |
| 25 | LD | + | − | Balanced | Slightly positive | 1.0 (1.0-1.0) | Intact |
LR indicates lymphocyte-rich; MC, mixed cellularity; NS, nodular sclerosis; LD, lymphocyte-depleted, NE: not evaluable, ND: not done; −, negative staining; +, staining in up to 30% of HRS cells; ++, staining in more than 30% and up to 70% of HRS cells; +++; staining in more than 70% of HRS cells; —, not applicable.
CGH data for cases 1 through 23 and 25 have been previously published. For comparison of the complete CGH karyotype, case numbers 1 through 23 and 25 correspond to case numbers in Joos et al8as follows: 2, 3, 4, 5, 6, 8, 13, 14, 15, 17, 19, 20, 21, 23, 24, 29a, 29b, 30, 31, 33, 34, 36, 37, 38, 40. Case cHL24 had the following CGH karyotype: rev ish enh(1q21-q44,4q32-q35,9,12q23-q24,17,20,21,22,Xq12-q28) dim(5q11-q35,11,13,14,16q21-q23) amp(2p13-p16).
The number of signals for the REL probe divided by the number of signals for the centromere of chromosome 2. Hybridization signals were scored in c- Rel+ HRS cells or in large atypical aneuploid cells when c-Rel expression was not detected.
Done by FISH.