VLA-4, VLA-5, and LFA-1 expression levels in synchronized CD34+ cells
| Cycle phase . | Mean channel fluorescence ratio . | ||
|---|---|---|---|
| VLA-4 . | VLA-5 . | LFA-1 . | |
| G0/G1 | 15.1 ± 5.6 | 1.1 ± 0.05 | 31.0 ± 0.1 |
| G1/S | 29.2 ± 8.4 | 8.4* ± 0.6 | 46.3* ± 1.8 |
| S | 36.0* ± 9.9 | 9.8* ± 0.4 | 37.7* ± 0.8 |
| Postmitotic phase | 38.5* ± 5.9 | 10.2* ± 0.9 | 82.1* ± 3.0 |
| Cycle phase . | Mean channel fluorescence ratio . | ||
|---|---|---|---|
| VLA-4 . | VLA-5 . | LFA-1 . | |
| G0/G1 | 15.1 ± 5.6 | 1.1 ± 0.05 | 31.0 ± 0.1 |
| G1/S | 29.2 ± 8.4 | 8.4* ± 0.6 | 46.3* ± 1.8 |
| S | 36.0* ± 9.9 | 9.8* ± 0.4 | 37.7* ± 0.8 |
| Postmitotic phase | 38.5* ± 5.9 | 10.2* ± 0.9 | 82.1* ± 3.0 |
VLA-4 (n = 8), VLA-5 (n = 3), and LFA-1 (n = 3) expression was determined by fluorescence-activated cell sorter (FACS) analysis at indicated time points during CD34+ cell synchronization cultures.
Expression of indicated receptor was statistically higher (P < .05) compared with freshly isolated G0/G1 CD34+ cells.