Effect of aphidicolin on the clonogenic activity of CD34+ cells
| Test cells . | Clonogenic activity . | |
|---|---|---|
| CFCs . | LTC-ICs . | |
| Freshly isolated cells | 53.5 ± 15.1 | 165.8 ± 17.9 |
| Aphidicolin cultures | 45.1 ± 11.4 | 174.2 ± 13.9 |
| Control cultures | 59.1 ± 2.3 | 157.5 ± 11.5 |
| Test cells . | Clonogenic activity . | |
|---|---|---|
| CFCs . | LTC-ICs . | |
| Freshly isolated cells | 53.5 ± 15.1 | 165.8 ± 17.9 |
| Aphidicolin cultures | 45.1 ± 11.4 | 174.2 ± 13.9 |
| Control cultures | 59.1 ± 2.3 | 157.5 ± 11.5 |
CFC and bulk LTC-IC assays were set up with freshly isolated CD34+ cells or with cells harvested from 40-hour ex vivo cultures supported by SCF, FL, and TPO in the presence or absence of aphidicolin for the last 24 hours. CFC data are expressed per 100 cells plated. LTC-IC activity is expressed as the number of secondary CFCs per 1000 input cells (n = 4).