Analysis of LIBS expression in Osaka-12 αllbβ3
| . | PMI-1 . | AP5 . | LIBS1 . | LIBS6 . | ||||
|---|---|---|---|---|---|---|---|---|
| . | (−) . | +FK633 . | (−) . | +FK633 . | (−) . | +FK633 . | (−) . | +FK633 . |
| Control | 1.53 ± 0.63 | 10.12 ± 4.14 | 0.95 ± 0.56 | 20.32 ± 7.78 | 16.59 ± 2.38 | 102.2 ± 14.66 | 2.24 ± 0.80 | 21.51 ± 1.53 |
| Osaka-12 | 4.3 ± 0.37 | 3.82 ± 0.77 | 5.59 ± 0.57 | 7.61 ± 0.53 | 12.15 ± 1.64 | 14.49 ± 4.96 | 7.36 ± 1.12 | 9.44 ± 0.43 |
| . | PMI-1 . | AP5 . | LIBS1 . | LIBS6 . | ||||
|---|---|---|---|---|---|---|---|---|
| . | (−) . | +FK633 . | (−) . | +FK633 . | (−) . | +FK633 . | (−) . | +FK633 . |
| Control | 1.53 ± 0.63 | 10.12 ± 4.14 | 0.95 ± 0.56 | 20.32 ± 7.78 | 16.59 ± 2.38 | 102.2 ± 14.66 | 2.24 ± 0.80 | 21.51 ± 1.53 |
| Osaka-12 | 4.3 ± 0.37 | 3.82 ± 0.77 | 5.59 ± 0.57 | 7.61 ± 0.53 | 12.15 ± 1.64 | 14.49 ± 4.96 | 7.36 ± 1.12 | 9.44 ± 0.43 |
Washed platelets were incubated with or without 10 μM FK633 (αllbβ3-specific antagonist), and then each anti-LIBS antibody was added. After washing, bound antibodies were detected by FITC-conjugated goat F(ab′)2 antimouse IgG. Results are expressed as mean fluorescence intensity (MFI) ± SD of triplicate measurements. The MFI for MOPC21 used as a negative control was subtracted. Results are representative of 2 separate experiments.