Table 1. Primers used for SSCP and... | American Society of Hematology

Table 1.

Primers used for SSCP and direct sequencing analysis

Forward primers^*	Reverse primers^*	Hybridization temperature, °C	Size of PCR product, bp	Restriction enzyme used (sizes of products after digestion, bp)
(EX1S) 5′-GTTGGGCTGGTGGAGGAGT-3′	(EX1AS) 5′-GACACCCCAAGGGGACTATC-3′	64	310	N/A
(EX2S) 5′-CTCTCTCCAGACCCCAGGA-3′	(EX2AS) 5′-CCTGACACCTCCTCTCTCA-3′	64	190	N/A
(EX3S) 5′-ACAGGCCTGGCAGTCTC-3′	(EX3AS) 5′-CTGAGCTCACCCCATACTG-3′	64	270	N/A
(EX4S) 5′-TGGCTGCTGGGTCAGTT-3′	(EX4AS) 5′-CTTCCTCCAGTCTCCTGTGC-3′	60	400	RsaI (180 and 220)
(EX5S) 5′-GGCGAGGGCTTAATGCTAT-3′	(EX5AS) 5′-GGGGCGGCTCCCATGTGG-3′	60	440	AluI (140 and 300)

Forward primers^*	Reverse primers^*	Hybridization temperature, °C	Size of PCR product, bp	Restriction enzyme used (sizes of products after digestion, bp)
(EX1S) 5′-GTTGGGCTGGTGGAGGAGT-3′	(EX1AS) 5′-GACACCCCAAGGGGACTATC-3′	64	310	N/A
(EX2S) 5′-CTCTCTCCAGACCCCAGGA-3′	(EX2AS) 5′-CCTGACACCTCCTCTCTCA-3′	64	190	N/A
(EX3S) 5′-ACAGGCCTGGCAGTCTC-3′	(EX3AS) 5′-CTGAGCTCACCCCATACTG-3′	64	270	N/A
(EX4S) 5′-TGGCTGCTGGGTCAGTT-3′	(EX4AS) 5′-CTTCCTCCAGTCTCCTGTGC-3′	60	400	RsaI (180 and 220)
(EX5S) 5′-GGCGAGGGCTTAATGCTAT-3′	(EX5AS) 5′-GGGGCGGCTCCCATGTGG-3′	60	440	AluI (140 and 300)

EX indicates exon, followed by exon number; S, sense; and AS, antisense.