Gene expression profiles in individual cells from defined CD34+ subpopulations circulating in neonatal cord blood
| Gene . | Peripheral blood CD34+ cell subset . | |||
|---|---|---|---|---|
| CD34+CD19−CD10−% . | CD34+CD19−CD10+% . | CD34+CD19+CD10−% . | CD34+CD19+CD10+ % . | |
| GAPDH | 97.2 (210 of 216) | 98.6 (213 of 216) | 100 (216 of 216) | 98.6 (213 of 216) |
| VpreB | 0 (0 of 210) | 0 (0 of 213) | 58.3 (126 of 216) | 54.9 (117 of 213) |
| TdT | 0 (0 of 210) | 0 (0 of 213) | 91.6 (198 of 216) | 54.9 (117 of 213) |
| RAG-1 | 0 (0 of 210) | 0 (0 of 213) | 37.5 (81 of 216) | 38 (81 of 213) |
| mb-1 | 3.3 (7 of 210) | 19.2 (41 of 213) | 51.3 (111 of 216) | 53.5 (114 of 213) |
| preTα | 1.4 (3 of 210) | 4.2 (9 of 213) | 0 (0 of 216) | 0 (0 of 213) |
| Gene . | Peripheral blood CD34+ cell subset . | |||
|---|---|---|---|---|
| CD34+CD19−CD10−% . | CD34+CD19−CD10+% . | CD34+CD19+CD10−% . | CD34+CD19+CD10+ % . | |
| GAPDH | 97.2 (210 of 216) | 98.6 (213 of 216) | 100 (216 of 216) | 98.6 (213 of 216) |
| VpreB | 0 (0 of 210) | 0 (0 of 213) | 58.3 (126 of 216) | 54.9 (117 of 213) |
| TdT | 0 (0 of 210) | 0 (0 of 213) | 91.6 (198 of 216) | 54.9 (117 of 213) |
| RAG-1 | 0 (0 of 210) | 0 (0 of 213) | 37.5 (81 of 216) | 38 (81 of 213) |
| mb-1 | 3.3 (7 of 210) | 19.2 (41 of 213) | 51.3 (111 of 216) | 53.5 (114 of 213) |
| preTα | 1.4 (3 of 210) | 4.2 (9 of 213) | 0 (0 of 216) | 0 (0 of 213) |
Indicated IgM−CD34+ hemopoietic progenitor populations were purified from neonatal cord blood from 8 donors, as shown in Figure 1. The correlated expression of the gene panel, measured at the single-cell level, was performed as in Figure 2 (18-36 cells/donor). Results indicate the percentage of individual cells showing amplification of the indicated genes among the total number of single cells analyzed in each subpopulation. Only tubes showing GAPDH amplification (97.2%-100%) were considered in this study. Numbers in parentheses indicate the number of cells showing specific amplification divided by the number of GAPDH+ cells analyzed in each subset.