Lentiviral transduction of non-T CD4+ cells
. | n . | LvPGK, % . | LvCD4, % . | LvCD4-Sil, % . |
---|---|---|---|---|
Dendritic cells | ||||
HLA-DR+CD4+ | 4 | 18.2 ± 4.5 | 25.7 ± 5.6 | 13.2 ± 3.1 |
Macrophages | ||||
CD14+CD4+ | 3 | 39.0 ± 20.1 | 36.0 ± 11.0 | 19.5 ± 2.0 |
. | n . | LvPGK, % . | LvCD4, % . | LvCD4-Sil, % . |
---|---|---|---|---|
Dendritic cells | ||||
HLA-DR+CD4+ | 4 | 18.2 ± 4.5 | 25.7 ± 5.6 | 13.2 ± 3.1 |
Macrophages | ||||
CD14+CD4+ | 3 | 39.0 ± 20.1 | 36.0 ± 11.0 | 19.5 ± 2.0 |
Frequencies of eGFP-expressing cells (± SD) within dendritic cells (HLA-DR+CD4+) and macrophages (CD14+CD4+) on transduction with the indicated lentiviral vectors. Non-T-cell subsets were derived in vitro from transduced CD34+ cells and analyzed for eGFP expression at day 12 of culture.