Lentiviral vector titers and concentrations
| . | LvPGK, n = 3 . | LvCD4, n = 4 . | LvCD4-Sil, n = 4 . |
|---|---|---|---|
| Unconcentrated, ip/mL | 106 ± 4.9 × 105 | 1.8 × 106 ± 1.2 × 106 | 1.8 × 106 ± 1.5 × 106 |
| Concentrated, ip/mL | 4.7 × 107 ± 1.7 × 107 | 6 × 107 ± 3 × 107 | 5.3 × 107 ± 4 × 107 |
| Fold enrichment (range) | 57.6 (18-109) | 44.6 (18-105) | 70 (12-216) |
| . | LvPGK, n = 3 . | LvCD4, n = 4 . | LvCD4-Sil, n = 4 . |
|---|---|---|---|
| Unconcentrated, ip/mL | 106 ± 4.9 × 105 | 1.8 × 106 ± 1.2 × 106 | 1.8 × 106 ± 1.5 × 106 |
| Concentrated, ip/mL | 4.7 × 107 ± 1.7 × 107 | 6 × 107 ± 3 × 107 | 5.3 × 107 ± 4 × 107 |
| Fold enrichment (range) | 57.6 (18-109) | 44.6 (18-105) | 70 (12-216) |
Lentiviral vectors were produced by cotransfection of 293T cells with 5 μg VSV-G envelope plasmid, 10 μg packaging plasmid pCMV · R8.91, and 10 μg transfer vectors. The table shows the comparison in titers of concentrated versus nonconcentrated lentiviral vectors supernatants on CEM × 174 cells. Titers of lentiviral vectors (ip/mL) ± SD were calculated as described in “Materials and methods.” Enrichment in viral titers was calculated as the ratio between lentiviral titers before and concentration (range).