Table 1. Cytokine production by MLR CD4... | American Society of Hematology

Table 1.

Cytokine production by^MLRCD4⁺ T cells

Stimuli	Cells	IL-2	IL-4	IL-10	TGF-β1
Allogeneic TCD PBMCs	Pre-G CD4⁺	30 (22-38)^*	< 10^*	80 (40-190)^*	3800 (3430-4500)^*
		6% (3%-7%)^†	2% (1%-2.5%)^†	2% (1.5%-3.5%)^†	6% (4%-7%)^†
Allogeneic TCD PBMCs	Post-G CD4⁺	< 10^*,^‡	< 10^*	178 (90-250)^*,^‡	3040 (2200-4075)^*
		< 1%^†,^‡	< 1%^†,^‡	12% (8%-18%)^†,^‡	5% (4%-8%)^†

Stimuli	Cells	IL-2	IL-4	IL-10	TGF-β1
Allogeneic TCD PBMCs	Pre-G CD4⁺	30 (22-38)^*	< 10^*	80 (40-190)^*	3800 (3430-4500)^*
		6% (3%-7%)^†	2% (1%-2.5%)^†	2% (1.5%-3.5%)^†	6% (4%-7%)^†
Allogeneic TCD PBMCs	Post-G CD4⁺	< 10^*,^‡	< 10^*	178 (90-250)^*,^‡	3040 (2200-4075)^*
		< 1%^†,^‡	< 1%^†,^‡	12% (8%-18%)^†,^‡	5% (4%-8%)^†

Ranges appear in parentheses.

Pre-G and post-G CD4⁺ T cells from the same donor were activated with allogeneic TCD PBMCs for 7 days in MLR at a 1:3 stimulator-responder ratio. After culture, IL-2, IL-4, IL-10, and TGF-β1 levels were measured by ELISA in culture supernatants, whereas the frequency of cytokine-producing CD4⁺ T cells was determined by flow cytometry after short-term exposure to a protein transport inhibitor (see “Patients, materials, and methods”). Binding specificities of the anticytokine mAb were established by adding recombinant cytokines before mAb staining (data not shown). All determinations were performed in triplicate. Data were reported as median values and ranges and are representative of 6 independent experiments.

pg/mL.

†

Percentage positive cells.

‡

P < .05 compared with pre-G^MLRCD4⁺ T cells.

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