Expression of cytokine and chemokine mRNA in PBMC subpopulations
| Patient no. (Days after transplantation) . | Subset . | Relative transcripts (cytokines or chemokines/GAPDH) . | |||||
|---|---|---|---|---|---|---|---|
| IL-10 . | IFN-γ . | IL-2 . | MIP-1α . | RANTES . | IP-10 . | ||
| 1 | CD4 | 2.28 | 3.01 | 0.05 | 6.44 | 29.59 | 357.14 |
| Day 33 | CD8 | 1.31 | 29.59 | 0.42 | 236.97 | 1086.96 | 126.58 |
| Monocyte | 4.25 | 6.01 | — | 236.97 | 558.66 | 256.41 | |
| 2 | CD4 | 0.55 | 0.11 | 0.01 | 20.96 | NT | 14.8 |
| Day 13 | CD8 | 0.25 | 2.44 | 0.02 | 78.13 | NT | — |
| Monocyte | 0.65 | 0.01 | — | 52.91 | NT | 52.43 | |
| 3 | CD4 | 1.14 | 0.05 | — | 40.81 | NT | 36.5 |
| Day 13 | CD8 | 0.68 | 0.71 | — | 20.65 | NT | — |
| Monocyte | 1.71 | 0.22 | — | 96.15 | NT | 90.1 | |
| 4 | CD4 | 0.15 | 0.25 | — | 7.05 | NT | 166.67 |
| Day 26 | CD8 | — | 29.85 | — | 46.73 | NT | — |
| Monocyte | — | 18.18 | — | 270.27 | NT | 833.33 | |
| 5 | CD4 | 0.07 | 0.05 | 0.01 | 4.46 | 1.62 | 41.8 |
| Day 13 | CD8 | 0.07 | 1.79 | 0.04 | 4.78 | 17.99 | 20.9 |
| Monocyte | 0.09 | 0.01 | — | 12.19 | 1.19 | 47.4 | |
| 6 | CD4 | 2.62 | 0.31 | 0.06 | 126.6 | 63.3 | 20.9 |
| Day 19 | CD8 | 0.53 | 10.47 | — | 22.5 | 1538.46 | 14.8 |
| Monocyte | 2.44 | 0.07 | 0.04 | 68.1 | 97.1 | 40.3 | |
| 7 | CD4 | 4.88 | 1.85 | 0.01 | NT | NT | NT |
| Day 26 | CD8 | — | 41.84 | 0.05 | NT | NT | NT |
| MonocyteT | Too few cells to evaluate | ||||||
| Patient no. (Days after transplantation) . | Subset . | Relative transcripts (cytokines or chemokines/GAPDH) . | |||||
|---|---|---|---|---|---|---|---|
| IL-10 . | IFN-γ . | IL-2 . | MIP-1α . | RANTES . | IP-10 . | ||
| 1 | CD4 | 2.28 | 3.01 | 0.05 | 6.44 | 29.59 | 357.14 |
| Day 33 | CD8 | 1.31 | 29.59 | 0.42 | 236.97 | 1086.96 | 126.58 |
| Monocyte | 4.25 | 6.01 | — | 236.97 | 558.66 | 256.41 | |
| 2 | CD4 | 0.55 | 0.11 | 0.01 | 20.96 | NT | 14.8 |
| Day 13 | CD8 | 0.25 | 2.44 | 0.02 | 78.13 | NT | — |
| Monocyte | 0.65 | 0.01 | — | 52.91 | NT | 52.43 | |
| 3 | CD4 | 1.14 | 0.05 | — | 40.81 | NT | 36.5 |
| Day 13 | CD8 | 0.68 | 0.71 | — | 20.65 | NT | — |
| Monocyte | 1.71 | 0.22 | — | 96.15 | NT | 90.1 | |
| 4 | CD4 | 0.15 | 0.25 | — | 7.05 | NT | 166.67 |
| Day 26 | CD8 | — | 29.85 | — | 46.73 | NT | — |
| Monocyte | — | 18.18 | — | 270.27 | NT | 833.33 | |
| 5 | CD4 | 0.07 | 0.05 | 0.01 | 4.46 | 1.62 | 41.8 |
| Day 13 | CD8 | 0.07 | 1.79 | 0.04 | 4.78 | 17.99 | 20.9 |
| Monocyte | 0.09 | 0.01 | — | 12.19 | 1.19 | 47.4 | |
| 6 | CD4 | 2.62 | 0.31 | 0.06 | 126.6 | 63.3 | 20.9 |
| Day 19 | CD8 | 0.53 | 10.47 | — | 22.5 | 1538.46 | 14.8 |
| Monocyte | 2.44 | 0.07 | 0.04 | 68.1 | 97.1 | 40.3 | |
| 7 | CD4 | 4.88 | 1.85 | 0.01 | NT | NT | NT |
| Day 26 | CD8 | — | 41.84 | 0.05 | NT | NT | NT |
| MonocyteT | Too few cells to evaluate | ||||||
CD4+ cells, CD8+ cells, and monocytes were separated from PBMCs of 7 patients who received CsA following transplantation. Patients no. 6 and 7 developed clinical GVHD. Monocytes were isolated by plastic adherence and gentle scraping. Lymphocytes were also separated into distinct T-cell subsets with immunomagnetic beads. — indicates that CT (threshold cycle) could not be detected; NT, not tested. Relative transcripts were determined as follows: 1/(CTCYTOKINE − CTGAPDH) 2 × 104.