Methylcellulose colony assay using 14.5-dpc fetal liver
| Cell population . | BFU-E (erythroid) . | CFU-GM (granulocyte/macrophage) . | CFU-GEM (mixed) . |
|---|---|---|---|
| Unsorted fetal liver | |||
| Cbfb+/+ | 5.0 ± 1.0 | 21.5 ± 1.5 | 2.0 ± 0 |
| Cbfb+/GFP | 4.5 ± 0.5 | 25.0 ± 0 | 3.0 ± 1.0 |
| CbfbGFP/GFP | 3.5 ± 0.5 | 19.0 ± 1.0 | 3.5 ± 0.5 |
| Sorted Cbfb+/GFP fetal liver | |||
| c-kit+/GFP+ | 42.0 ± 11.1 | 153.0 ± 28.2 | 26.2 ± 6.7 |
| c-kit+/GFP− | 6.3 ± 2.9 | 43.3 ± 3.1 | 6.0 ± 3.6 |
| Cell population . | BFU-E (erythroid) . | CFU-GM (granulocyte/macrophage) . | CFU-GEM (mixed) . |
|---|---|---|---|
| Unsorted fetal liver | |||
| Cbfb+/+ | 5.0 ± 1.0 | 21.5 ± 1.5 | 2.0 ± 0 |
| Cbfb+/GFP | 4.5 ± 0.5 | 25.0 ± 0 | 3.0 ± 1.0 |
| CbfbGFP/GFP | 3.5 ± 0.5 | 19.0 ± 1.0 | 3.5 ± 0.5 |
| Sorted Cbfb+/GFP fetal liver | |||
| c-kit+/GFP+ | 42.0 ± 11.1 | 153.0 ± 28.2 | 26.2 ± 6.7 |
| c-kit+/GFP− | 6.3 ± 2.9 | 43.3 ± 3.1 | 6.0 ± 3.6 |
Cells were isolated from fetal liver of 14.5-dpcCbfb+/+,Cbfb+/GFP, andCbfbGFP/GFP embryos. Cells fromCbfb+/GFP embryos were sorted into c-kit+/GFP+ and c-kit+/GFP− populations by FACS (Figure 4C). Progenitors in each of these populations (sorted and unsorted) were assessed by methylcellulose colony assay. The table shows the average data collected from cultures of unsorted cells (fromCbfb+/+,Cbfb+/GFP,CbfbGFP/GFP) and sorted cells (n = 3); 1 × 104 fetal liver cells were plated in each culture.