Methylcellulose colony assay using adult bone marrow
| Cell population . | BFU-E (erythroid) . | CFU-GM (granulocyte/macrophage) . | CFU-GEM (mixed) . |
|---|---|---|---|
| Unsorted bone marrow | 16.7 ± 2.4 | 68 ± 11.8 | 10 ± 4.1 |
| GFP+ | 35 ± 10.8 | 125 ± 10.8 | 28.3 ± 6.2 |
| GFP− | 3.3 ± 2.4 | 5 ± 4.1 | 0 |
| Cell population . | BFU-E (erythroid) . | CFU-GM (granulocyte/macrophage) . | CFU-GEM (mixed) . |
|---|---|---|---|
| Unsorted bone marrow | 16.7 ± 2.4 | 68 ± 11.8 | 10 ± 4.1 |
| GFP+ | 35 ± 10.8 | 125 ± 10.8 | 28.3 ± 6.2 |
| GFP− | 3.3 ± 2.4 | 5 ± 4.1 | 0 |
Progenitors in all 3 populations (unsorted heterozygous bone marrow, GFP+, and GFP−) shown in Figure 3B were assessed by methylcellulose colony assay. The table shows the mean and SD of the data collected from 3 independent experiments (n = 3); 5 × 104 cells from each population were plated in each culture.