Production of CC chemokines by early- and late-lineage B cells from bone marrow
| Sample . | CCL3 secretion (pg/mL ± SD) . | CCL4 secretion (pg/mL ± SD) . | CCL5 secretion (pg/mL ± SD) . | CCL2 secretion (pg/mL ± SD) . | CCL11 secretion (pg/mL ± SD) . |
|---|---|---|---|---|---|
| Medium | < dt | < dt | < dt | < dt | < dt |
| Early-lineage B cells | 230 ± 298 | 134 ± 189 | < dt | 70 ± 36 | < dt |
| Late-lineage B cells | 492 ± 354 | 1192 ± 341 | < dt | 156 ± 35 | < dt |
| Sample . | CCL3 secretion (pg/mL ± SD) . | CCL4 secretion (pg/mL ± SD) . | CCL5 secretion (pg/mL ± SD) . | CCL2 secretion (pg/mL ± SD) . | CCL11 secretion (pg/mL ± SD) . |
|---|---|---|---|---|---|
| Medium | < dt | < dt | < dt | < dt | < dt |
| Early-lineage B cells | 230 ± 298 | 134 ± 189 | < dt | 70 ± 36 | < dt |
| Late-lineage B cells | 492 ± 354 | 1192 ± 341 | < dt | 156 ± 35 | < dt |
Early- (pro-B and pre-B cells combined) and late- (immature and mature B cells combined) lineage B cells sorted from bone marrow were cultured for 24 hours in serum-free medium at a concentration of 106 cells/mL medium. The detection threshold of the enzyme-linked immunosorbent assays for CCL3, CCL5, and CCL2 was greater than 31 pg/mL, and for CCL4 and CCL11 more than 15 pg/mL. The means ± SD of 3 experiments, each done in duplicate, are presented. < dt indicates below the threshold of detection.