Reduced factor-independent colony formation by BCR-ABL-transduced IL-3−/− LTC-IC-derived CFCs is restored when the cells are cotransduced with an IL-3 cDNA
| Cells . | Virus . | No. of LTC-IC–derived CFCs detected (per 103 input cells) . | |||
|---|---|---|---|---|---|
| BFU-E . | CFU-GEMM . | CFU-GM . | Total . | ||
| −/− | IL-3 | 75 ± 15 | 37 ± 12 | 690 ± 30 | 800 ± 60 |
| +/+ | IL-3 | 90 ± 12 | 45 ± 30 | 800 ± 57 | 830 ± 70 |
| −/− | BCR-ABL | 60 ± 12 | 40 ± 15 | 650 ± 60 | 760 ± 60 |
| +/+ | BCR-ABL | 120 ± 20 | 70 ± 30 | 1750 ± 150 | 1940 ± 200 |
| −/− | IL-3 + BCR-ABL | 140 ± 20 | 70 ± 30 | 1830 ± 150 | 2040 ± 200 |
| +/+ | IL-3 + BCR-ABL | 250 ± 30 | 140 ± 20 | 2235 ± 115 | 2625 ± 110 |
| Cells . | Virus . | No. of LTC-IC–derived CFCs detected (per 103 input cells) . | |||
|---|---|---|---|---|---|
| BFU-E . | CFU-GEMM . | CFU-GM . | Total . | ||
| −/− | IL-3 | 75 ± 15 | 37 ± 12 | 690 ± 30 | 800 ± 60 |
| +/+ | IL-3 | 90 ± 12 | 45 ± 30 | 800 ± 57 | 830 ± 70 |
| −/− | BCR-ABL | 60 ± 12 | 40 ± 15 | 650 ± 60 | 760 ± 60 |
| +/+ | BCR-ABL | 120 ± 20 | 70 ± 30 | 1750 ± 150 | 1940 ± 200 |
| −/− | IL-3 + BCR-ABL | 140 ± 20 | 70 ± 30 | 1830 ± 150 | 2040 ± 200 |
| +/+ | IL-3 + BCR-ABL | 250 ± 30 | 140 ± 20 | 2235 ± 115 | 2625 ± 110 |
LTC-IC assays were initiated with 103lin− cells from each population. The LTCs were harvested after 4 weeks and the cells in them were then assayed for CFCs in FCS-containing methylcellulose medium in the absence of added growth factors. Data shown are the mean ± SEM of values obtained from 3 independent experiments. LTC-IC indicates long-term culture-initiating cell; CFC, colony-forming cell; IL-3, interleukin 3; BFU-E, erythroid-burst-forming unit; CFU-GEMM, colony-forming unit–granulocyte/erythroid/macrophage/megakaryocyte; CFU-GM, granulocyte macrophage-colony-forming unit; FCS, fetal calf serum.