Frequency of donor cells detected in BM, spleen, and PB of irradiated mice 3 hours after transplantation is positively correlated with the number of graft cells injected
*Cell dose . | 104 . | 105 . | 106 . | 107 . | 108 . | r2 . |
---|---|---|---|---|---|---|
BM | < 0.01† | < 0.01 | 0.05 | 3.30 | 24.94 | 0.999‡ |
Spleen | < 0.01 | 0.03 | 0.33 | 5.31 | 28.48 | 0.992 |
PB | < 0.01 | < 0.01 | 1.47 | 1.88 | 29.06 | 0.996 |
*Cell dose . | 104 . | 105 . | 106 . | 107 . | 108 . | r2 . |
---|---|---|---|---|---|---|
BM | < 0.01† | < 0.01 | 0.05 | 3.30 | 24.94 | 0.999‡ |
Spleen | < 0.01 | 0.03 | 0.33 | 5.31 | 28.48 | 0.992 |
PB | < 0.01 | < 0.01 | 1.47 | 1.88 | 29.06 | 0.996 |
BM indicates bone marrow; PB, peripheral blood.
Number of PKH26+ syngeneic low-density BM cells injected into single irradiated recipient mice.
Numbers represent the percent of donor graft cells detected by flow cytometry in BM, spleen, and PB at 3 hours after transplantation. The lower limit of detection was 0.01%; n = 1 for each value.
Correlation coefficient of linear regression analysis of cell dose and percent of donor cells detected in BM, spleen, and PB.